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Cadmium appears to be the largest single contributor to
autoimmune thyroid disease. It is a very powerful and toxic metal which
seems to be placed at the very center of the thyroid story. I think you'll
find this file very interesting.
Not only does cadmium appear to play a very pivotal role in
thyroid disease, it is a very unique mineral. It is extremely toxic and
has toxic biological effects at concentrations smaller than almost any commonly
Despite this great toxicity, there is some evidence that
cadmium is an essential nutrient with biological function. We will be
exploring this dichotomy of cadmium.
One of the greatest effects of cadmium is that it depletes
selenium in the body because selenium is essential for cadmium removal. Selenium
atoms combine with cadmium atoms and are escorted out of the body via the bile
system. When selenium is depleted by cadmium, there is less selenium to form the
deiodinase enzymes which convert T4 to T3, resulting in low T3 and
hypothyroidism. Also there is less selenium to form glutathione peroxidase, one
of the body's prime antioxidants. This results in greater levels of reactive
oxygen species and hydrogen peroxide, which lead to an increased production of
thyroid hormone and damage to the thyroid gland.
The following study shows that cadmium and mercury
toxicities (at high levels) will induce immediate hyperthyroidism.
Thyrotoxicity of the chlorides of cadmium and mercury
Ghosh N; Bhattacharya S
Department of Zoology, Visva-Bharati University, Santiniketan,
Biomed Environ Sci, 5(3):236-40 1992 Sep
Exposure to heavy metals such as cadmium and
mercury is of immediate environmental concern. The present study was
aimed at establishing a direct relationship between heavy metal poisoning and
thyroid dysfunction. Cadmium and mercury treatment at LD50 levels
resulted in severe thyrotoxicosis in the rabbit. Within 24 h of
intramuscular administration of cadmium chloride 15 mg.kg-1 body weight
(bw) and mercury chloride 20 mg.kg-1 bw, thyroid peroxidase activity increased
significantly over the control with a concomitant rise in the
triiodothyronine (T3) titre. On the other hand, there was a remarkable
fall in the thyroxine (T4) level, and the T3/T4 ratio was high as compared
with the control. Evidence indicates that acute heavy metal lethality will
induce immediate hyperthyroidism. It is suggested that T3-toxicosis may be
produced by a preferential synthesis of T3 and/or preferential deiodination of
T4 to T3. Measurement of T3 and T4 levels may thus be utilized as a
reliable indicator of heavy metal lethality.
The following study examines the thyroidal functioning of
inhabitants of a cadmium polluted river basin in Japan. Free T4 levels are
found to be lower than normals but T3 levels are higher.
[A study of thyroid hormone levels of inhabitants of the cadmium-polluted
Kakehashi River basin]
Nishijo M; Nakagawa H; Morikawa Y; Tabata M; Senma M; Miura
K; Tsuritani I; Honda R; Kido T; Teranishi H; et al
Department of Public Health, Kanazawa Medical University,
Nippon Eiseigaku Zasshi, 49(2):598-605 1994 Jun
We compared thyroid hormone levels of inhabitants (19 men
and 16 women) of the cadmium (Cd)-polluted Kakehashi River basin in Ishikawa
Prefecture, with those of subjects (23 men and 47 women) living in a
non-polluted area. In addition, we investigated the relationships between the
thyroid hormone levels and indices of renal dysfunction induced by Cd
exposure. The following results were obtained: 1) The free T4 level of
females was significantly lower than that of controls. 2) The T3 level
of inhabitants of both sexes was significantly higher than that of controls.
3) The level of free T4 among females became lower with the increases of
urinary beta 2-microglobulin (beta 2-MG), urinary protein, urinary sugar,
urinary amino acids and serum creatinine (Cr) levels, and with decreases of
creatinine-clearance (CCr) and %TRP. 4) We could not find any relationship
between the increase of T3 and the indices of renal dysfunction induced by Cd
exposure in either sex.
- The following study shows that cadmium decreases T3 by interfering with
T4 to T3 conversion.
|Environ Res 1987 Apr;42(2):400-5
Effect of cadmium on T4 outer ring monodeiodination by rat
Yoshida K, Sugihira N, Suzuki M, Sakurada T, Saito S, Yoshinaga K, Saito H
The effect of cadmium on thyroxine (T4) outer ring monodeiodination was studied
in vivo and in vitro in the rat liver. One microgram of T4 was incubated with
rat liver homogenates in 50 mM Tris--HCl buffer, pH 7.4, with or without 0.5, 5,
and 50 mM dithiothreitol (DTT) for 60 min in the presence of 10(-8) to 10(-3) M
CdCl2, and the amount of 3,5,3'-triiodothyronine (T3) produced was determined by
a specific radioimmunoassay. Subcutaneous injection of CdCl2, 1 mg/kg BW/day, 5
days a week for 10 weeks, to the rats resulted in a significant reduction in
serum T3 concentration (by 37%) and hepatic T3 production from T4 (by 78 to
92%). In vitro addition of 1 microM to 1 mM CdCl2 to liver homogenates caused a
concentration-dependent reduction in T3 generation. Without DTT a 50% reduction
in the T4 to T3 converting activity was caused by 4 X 10(-6) M CdCl2. DTT (0.5
to 50 mM) partially restored T3 generation roughly in a concentration-dependent
manner. These results indicate that cadmium has some effects on the metabolism
of thyroid hormone.
The following study demonstrates the existence of a cadmium-binding protein (CdBP)
in human thyroid tissue but not in dogs, pigs, and oxen. Cadmium
accumulates in the thyroid, kidney, liver, and pancreas--all areas that seem to
be involved in thyroid disease.
|Toxicol Lett 1982 May;11(3-4):269-73
Cadmium-binding proteins in human organs.
Sato M, Takizawa Y
Cadmium-binding proteins (CdBPs) in the cytosol fractions from several
organs of man orally exposed to cadmium (Cd) were examined by gel
chromatography. In kidney and liver most of the cadmium (76-87%) in the
cytosols was bound to metallothionein, and hepatic metallothionein contained
zinc also at a similar level. The pancreas cytosol also contained a
metallothionein-like CdBP, although its content was only one-tenth of the
hepatic one. In the thyroid gland a prominent CdBP, eluting later than
that of metallothionein, was observed; this CdBP was not detectable in
normal dogs, pigs, and oxen. No CdBPs were observed in other organs
including heart, muscle, genital organs, aorta, and bone. These results
suggest that CdBPs participate in accumulation and distribution of cadmium
in man, since organs containing CdBP such as kidney, liver, pancreas, and
thyroid gland show a tendency to accumulate high levels of cadmium.
The following study indicates that cadmium may
inhibit lipogenesis by binding with the thiol group (SH) of coenzyme A,
thereby reducing the serum levels of free fatty acids and lipid peroxides.
Keep in mind that all antithyroid drugs all have this thiol group and
providing this may be their mode of action. Intriguingly, cadmium was
found to have different effects in males and females in regard to the effects
on fatty acids. This may be a possible mechanism to explain why females
get thyroid diseases at a much higher rate than males. Also interesting
is that cadmium by interfering with the action of the thiol group may be
interfering with the metabolism of vitamin D and possibly the conversion of
cholesterol into the steroid (sex) hormones. This may explain how
cadmium increases osteoporosis, a phenomenon demonstrated by osteoporosis in
older smokers, especially women.
[Effect of cadmium on lipid components: relation of cadmium
to thyroid hormone and growth hormone]
Department of Public Health, Hyogo College of Medicine,
Nippon Eiseigaku Zasshi, 47(3):704-14 1992 Aug
To clarify the relationship of cadmium (Cd), thyroid
hormone (TH) and growth hormone (GH) to lipid components, 4-week-old SD rats
were dosed orally with Cd (CdCl2) at a dose of 2.0 mg/kg body weight five
times a week, orally with TH at a dose of 2.5 mg/kg body weight five times a
week and subcutaneously with GH (somatotropin) at a dose of 1.0 IU/kg body
weight three times a week, all for 4 weeks. As lipid components, the serum
concentrations of triglycerides, free fatty acids, lipid peroxides and
long-chain fatty acids were determined. We have devised a new method for
determining the fatty acid composition in the femur using gas
chromatography-mass spectrometry and made a simultaneous analysis of fatty
acids, from myristic acid (C14:0) to cholesterol. The results of the present
study led to the following conclusions. 1. Cd may inhibit lipogenesis by
binding with SH of coenzyme A, thereby reducing the serum levels of free fatty
acids and lipid peroxides. 2. When TH and Cd were administered in
combination, the addition of Cd produced an inhibitory effect on lipid
components, although TH given alone stimulated the lipid metabolism.
Therefore, Cd and TH may have an interaction in lipid components. 3. When
GH and Cd were administered in combination, Cd modulated the action of GH,
which enhanced the effect of somatomedin on the lipid metabolism. The
inhibitory effect of Cd on somatomedin activity via Zn was suggested. 4. A
sex difference was found in the composition of fatty acids in blood. The males
had higher proportions of palmitic acid (C16:0) and linoleic acid (C18:2),
while the females had a higher proportion of arachidonic acid (C20:4).
There was no sex difference in fatty acid composition in the femur. 5. It
was confirmed that TH produced a peroxide of dehydrocholesterol, a precursor
of vitamin D3, in the diaphysis of the femur in the increased metabolic state.
The following study indicates how cadmium enters the food chain.
Cadmium waste from industrial production is disposed in the sewers and the
sewage sludge is used for agricultural fertilizer. Also there is an
indication that cadmium might be present in phosphate fertilizers since it is
used in their manufacture. If this is true then virtually all
non-organically grown foods are going to have higher than normal amounts of
Title: Sources of cadmium in the environment.
- Hutton M
- Ecotoxicol Environ Saf, 7(1):9-24 1983 Feb
- This paper is concerned with quantifying the major sources
of cadmium in the European Community and assessing the relative
significance of such inputs to the environmental compartments, air, land,
and water. The methodology involved identification of potential sources of cadmium,
including natural processes, as well as those associated with human
activities. This was followed by a review of any emission studies of these
processes and subsequent estimation of an emission factor for each source.
The emission factor was applied to the most recent production or consumption
data for the process in question to obtain an estimate of the annual
discharge. The steel industry and waste incineration, followed by volcanic
action and zinc production, are estimated to account for the largest
emissions of atmospheric cadmium in the region. Waste disposal
results in the single largest input of cadmium to land; the quantity
of cadmium associated with this source is greater than the total from the
four other major sources--coal combustion, iron and steel production,
phosphate fertilizer manufacture and use, and zinc production. The
characterization of cadmium inputs to aquatic systems is incomplete
but of the sources considered, the manufacture of cadmium-containing
articles accounts for the largest discharge, followed by phosphate
fertilizer manufacture and zinc production.
Arsenic-cadmium interaction in rats: toxic effects in
the heart and tissue metal shifts.
Y´a~nez L; Carrizales L; Zanatta MT; Mej´ia JJ; Batres L;
Departamento de Bioqu´imica, Facultad de Medicina,
Universidad Aut´onoma de San Luis Potos´i, Mexico.
Toxicology, 67(2):227-34 1991 Apr 8
Previously, we had shown that arsenic interacts with cadmium
in rats; our results showed that the toxicity of a mixture of arsenic + cadmium
cannot be predicted by the toxic mechanisms of the individual components. In
this paper, we present further evidence about the interaction of arsenic and cadmium
in rats. The results were: arsenic modified the 24 h-LD50 value of cadmium
more clearly than cadmium did with the one of arsenic; based on the
LD50 values, the mixtures we studied were more toxic than either metal alone.
With single doses (As 10 mg/kg, Cd 2.6 mg/kg, and As 10 mg/kg + Cd 2.6 mg/kg)
the mixture As + Cd was more toxic than each metal. At these doses, cadmium
significantly induces the levels of glutathione, metallothionein, and lipid
peroxidation in heart tissue, as compared to a saline group of rats. Arsenic
incremented glutathione and lipid peroxidation at higher values than those
obtained with cadmium. The mixture of As + Cd behaved as arsenic in the
induction of lipid peroxidation and glutathione and like cadmium in
metallothionein induction. Finally, rats treated with As + Cd had less Cd in
liver than animals treated only with cadmium, and more As in heart
tissue than rats treated only with arsenic. Our results give further evidence
about the arsenic-cadmium interaction in rats, demonstrate the utility
of employing different biomarkers in the study of chemical mixtures and
indicate that heart tissue is affected not only by the mixture of As + Cd, but
also by either metal alone.
- To me the following study is another indication that cadmium is a selenium
antagonist. Since arsenic is just to the left of selenium in the
Periodic Table and is known to be a selenium antagonist, it's not surprising
that when arsenic and cadmium (also a selenium antagonist) are combined
there is an increased combined effect of reducing selenium and glutathione.
Cadmium interferes with steroid
biosynthesis in rat granulosa and luteal cells in vitro.
Paksy K; Varga B; L´az´ar P
National Institute of Occupational Health, Budapest, Hungary.
Biometals, 5(4):245-50 1992 Winter
Recently, cadmium has been described to disturb ovarian
function in rats. In this paper the direct influence of cadmium on
steroid production of ovarian cells in vitro has been studied. Granulosa and
luteal cells were obtained from proestrous and pregnant rats, and incubated
with 0, 5, 10, 20 or 40 micrograms ml-1 CdCl2 in the presence or absence of
0.1-1000 ng ml-1 follicle stimulating hormone (FSH) or luteinizing hormone (LH)
for 24 or 48 h. Production of progesterone (P) and 17 beta-estradiol (E2)
by granulosa and that of P by luteal cells were measured by radioimmunoassay.
In FSH-stimulated granulosa cell cultures, 5 and 40 micrograms ml-1 CdCl2
suppressed P accumulation to 65 and 10%, respectively; accumulation of E2 (at
5 micrograms ml-1 CdCl2) decreased to 44%. P production of LH-supported luteal
cells dropped to 86 and 66%, respectively, when 5 and 40 micrograms ml-1 CdCl2
was added to the medium. No alteration in basal P accumulation occurred
in granulosa and luteal cell cultures following incubations with 20 and 40
micrograms ml-1 CdCl2, whereas basal E2 production of granulosa cells was
markedly diminished. It is concluded that CdCl2 suppressing steroid
synthesis in vitro exerts a direct influence on granulosa and luteal cell
- The following study shows that while cadmium seems not to affect
progesterone levels it does decrease estrogen (estradiol) levels. My
suspicion is that cadmium accomplishes this by decreasing copper
levels and that copper is essential for the conversion of progesterone
The following study indicates that cadmium causes
emphysema, a disease characterized by extensive disruption of lung
connective tissue, by inhibiting the production of connective tissue
Cadmium inhibits proteoglycan and
procollagen production by cultured human lung fibroblasts.
Chambers RC; Laurent GJ; Westergren-Thorsson G
Centre for Cardiopulmonary Biochemistry and Respiratory
Medicine, University College Medical School, Rayne Institute, London, United
Am J Respir Cell Mol Biol, 19(3):498-506 1998 Sep
Chronic inhalation of cadmium at the workplace or in
cigarette smoke is associated with emphysema, a disease characterized by
extensive disruption of lung connective tissue. We have previously shown that
cadmium, at noncytotoxic doses, inhibits fibroblast procollagen production in
vitro, with maximal inhibitory effects of 69 +/- 6% (P < 0.01) at 30
µM cadmium chloride (CdCl2). In this paper we show that at similar
doses, cadmium also inhibits proteoglycan synthesis, with values
reduced by between 36 +/- 4% (P < 0.01) and 42 +/- 6% (P < 0.01) for
proteoglycans secreted into the culture media and associated with the cell
layer, respectively. The greatest inhibition was obtained for the major
matrix-associated proteoglycans, versican, decorin, and the large heparan
sulfate proteoglycans, with synthesis values reduced by between 60 and
70%. Biglycan and other heparan sulfate proteoglycans were also affected, with
synthesis values reduced by between 25 and 45%. In contrast, total protein
synthesis was unaffected. Furthermore, effects of cadmium at the
protein level were mirrored by reduction in messenger RNA levels for alpha1(I)
procollagen, versican, and decorin. These data support the hypothesis that
cadmium may play an important role in the pathogenesis of emphysema associated
with chronic inhalation of cadmium fumes by inhibiting the production of
connective tissue proteins.
The following study indicates that copper protects cells from cadmium
Protective effect of copper against cadmium
cytotoxicity on cultured vascular endothelial cells.
Kaji T; Fujiwara Y; Koyanagi E; Yamamoto C; Mishima A;
Sakamoto M; Kozuka H
Department of Environmental Science, Faculty of
Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Toxicol Lett, 63(1):13-20 1992 Oct
We investigated the effect of copper on cadmium-induced
cytotoxicity on vascular endothelial cells from bovine aorta in a culture
system. Cytotoxicity was evaluated by the [3H]adenine release assay and the
histological observation. After a 24-h incubation, cadmium exhibited a
significant cytotoxicity on confluent cultures of endothelial cells in a
dose-dependent manner, while copper only slightly did after a 24-h incubation.
It was found that copper (5 microM) significantly decreased cadmium (1
and 2 microM) cytotoxicity; histologically, formation of de-endothelialized
areas in the cell layer caused by cadmium was reduced by copper. The
accumulation of cadmium in the cell layer was significantly decreased
by copper; however, that of copper was unaffected by cadmium. It was
therefore suggested that copper significantly protects cadmium-induced
cytotoxicity on cultured endothelial cells primarily through decreasing the
cellular cadmium accumulation.
The following study is significant because it shows that alcohol increases
cadmium uptake into the body and have a combined stronger effect in decreasing
copper and zinc levels. This demonstrates the danger of combined smoking
and drinking in damaging the thyroid.
- Effect of ethanol on cadmium uptake and metabolism
of zinc and copper in rats exposed to cadmium.
- Sharma G; Sandhir R; Nath R; Gill K
- Department of Biochemistry, Postgraduate Institute, Medical
Education and Research, Chandigarh, India.
- J Nutr, 121(1):87-91 1991 Jan
- Effects of chronic administration of cadmium and
ethanol, alone as well as in combination, on the uptake of cadmium and
its interaction with other essential trace elements in various tissues
of adult rats were investigated. Cadmium given in combination with
ethanol led to a pronounced increase in cadmium absorption and accumulation in
all the tissues studied relative to both non-exposed controls and rats
treated with cadmium alone. Both cadmium and ethanol exhibited
specific effects on copper and zinc levels of the tissues. These effects
often were significantly altered when the animals were co-exposed to cadmium
and ethanol. The results suggested that although both cadmium and ethanol
individually pose a hazard to essential trace metal homeostasis of various
organs, co-exposure can pose a major threat since animals exposed to ethanol
absorb much more cadmium than their unexposed counterparts.
The following study suggests that the mechanism by which
cadmium antagonizes zinc may be from its ability to substitute for zinc in the
zinc finger DNA binding domain and this may be the way cadmium causes toxicity
Effect of replacement of "zinc finger zinc" on estrogen
receptor DNA interactions.
Predki PF; Sarkar B
Department of Biochemistry Research, Hospital for Sick
Children, Toronto, Ontario, Canada.
J Biol Chem, 267(9):5842-6 1992 Mar 25
Exposure of bovine estrogen receptor to the metal chelators
EDTA and 1,10-phenanthroline results in a loss of nonspecific DNA binding,
presumably because of the removal of "zinc finger zinc." Nonspecific DNA
binding, as measured by a DNA-cellulose binding assay, can be restored by
dialysis of the aporeceptor against buffer containing zinc, cadmium,
and cobalt but not with buffer containing copper or nickel. More detailed
studies were carried out using a bacterially expressed polypeptide
encompassing the DNA binding domain of the human estrogen receptor.
Apopolypeptide fails to bind DNA specifically, as measured by mobility shift
assay using a consensus estrogen response element hexamer containing
oligonucleotide, but DNA binding was restored by dialysis of the
apopolypeptide against buffer containing zinc, cadmium, and cobalt but
not with buffer containing copper or nickel. Dissociation constants of zinc-
and cadmium-reconstituted polypeptide for the estrogen response element
hexamer (66 and 48 nM, respectively) are virtually indistinguishable from
native polypeptide (Kd = 48 nM) whereas cobalt-reconstituted polypeptide has a
lower affinity (Kd = 720 nM). However, native, zinc-, cadmium-, and
cobalt-reconstituted polypeptides gave identical results in a methylation
interference assay. Competition experiments with zinc and copper or nickel
suggest that copper and nickel are able to bind to zinc finger residues but do
so nonproductively. The relative affinities copper greater than cadmium
greater than zinc greater than cobalt greater than nickel for the polypeptide
were determined by a zinc blot competition assay. The ability of cadmium
and cobalt to substitute for zinc in the zinc fingers demonstrates a
structural "flexibility in the DNA binding domain as each of these metals
has slightly different ionic radii. On the other hand, subtle differences in
DNA binding affinity and/or specificity could exist, which may not be
detectable here. Also, the ability of metals to substitute for zinc in the DNA
binding domain suggests that metal substitution in these zinc fingers in vivo
may be of relevance to the toxicity and/or carcinogenicity of some of these
Cadmium toxicity in the thyroid
gland of pregnant rats.
Yoshizuka M; Mori N; Hamasaki K; Tanaka I; Yokoyama M; Hara
K; Doi Y; Umezu Y; Araki H; Sakamoto Y; et al
Department of Anatomy, School of Medicine, University of
Occupational and Environmental Health, Kitakyushu, Japan.
Exp Mol Pathol, 55(1):97-104 1991 Aug
The toxic effects of cadmium on the thyroid gland of
pregnant rats were studied with an electron microscope and an X-ray
microanalyzer. Serum levels of thyroid hormones (T3 and T4) were also
analyzed. Deterioration of the rough-surfaced endoplasmic reticulum
occurred in the thyroid follicular epithelium on the fifth day of cadmium
treatment. Large intracellular vacuoles, which arose from dilated
cisternae of the rough-surfaced endoplasmic reticulum, were fused together,
and marked swelling of the mitochondria was also noted. Thyroglobulin-secreting
granules at the apical cytoplasm were decreased in number. By energy
dispersive X-ray microanalysis, cadmium peaks were preferentially
obtained from swollen mitochondria in the follicular epithelial cells.
Serum levels of T3 and T4 were significantly decreased in cadmium-treated rats
dams when compared to those of controls. In the present experiment,
cycloheximide also caused degenerative changes in the rough-surfaced
endoplasmic reticulum and the disappearance of thyroglobulin-secreting
granules. Cycloheximide is a known inhibitor of protein synthesis on cytosolic
ribosomes. These results indicated that accumulated cadmium in the
mitochondria of thyroid follicular epithelial cells might disturb the
oxidative phosphorylation of this organelle and the loss of energy supply
possibly caused the inhibition of the synthesis and release of thyroid
- The following study shows that cadmium damages the cells of
the thyroid, reduces thyroglobulin producing cells, and decreases both T4
Cadmium induced thyroid dysfunction in chicken: hepatic type I
iodothyronine 5'-monodeiodinase activity and role of lipid peroxidation.
Gupta P; Kar A
Thyroid Research Unit, School of Life Sciences, D.A. University, Vigyan
Bhawan, Indore, India.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol, 123(1):39-44 1999 May
Administration of cadmium chloride (2.5 mg/kg body weight/day) to
chickens daily for 15 days decreased serum triiodothyronine (T3)
concentration (by 68.75%) without altering the levels of serum thyroxine
(T4). Hepatic 5'-monodeiodinase (5'D-I) and superoxide dismutase
(SOD) activities were also decreased (by 90.47% and 20.81% respectively)
with a concomitant increase in lipid peroxidation (LPO, by 206.25%). Administration
of the antioxidant vitamin E (alpha-tocopherol, 5 mg/kg body weight on
alternate days) to cadmium intoxicated chickens restored thyroid function by
maintaining normal hepatic 5'D-I activity and serum thyroid hormone
concentrations. It also prevented cadmium-induced increase
in LPO. We conclude that the metal-induced inhibition in hepatic 5'D-I
activity is mediated through LPO.
- The following study shows that cadmium decreases T3,
probably by inhibiting hepatic 5'-monodeiodinase (5'D-I) activity, which is
a selenium dependent function. Cadmium is a known selenium antagonist
while vitamin E facilitates selenium metabolism. Vitamin E was shown
to protect against cadmium toxicity and maintain 5'D-I activity and T3
levels. While the experimenters concluded that "the metal-induced
inhibition in hepatic 5'D-I activity is mediated through LPO (lipid
peroxidation)" my conclusion is that the cadmium inhibited 5'D-I
activity by decreasing selenium. While vitamin E does decrease lipid
peroxidation, it does this by facilitating selenium metabolism and selenium
is the key metal in glutathione peroxidase which is a potent inhibitor of
Role of ascorbic acid in cadmium-induced thyroid dysfunction
and lipid peroxidation.
Gupta P; Kar A
Thyroid Research Unit, School of Life Sciences, Devi Ahilya University,
J Appl Toxicol, 18(5):317-20 1998 Sep-Oct
A study on the effects of ascorbic acid (AA) on heavy metal
(cadmium)-induced thyroid dysfunction and lipid peroxidation (LPO) was
carried out in Swiss male mice. The animals were administered with either cadmium
(1.0 mg kg(-1) body wt.) alone or in combination with AA (1 mM) every day
for 15 days. While cadmium treatment led to a decrease in the
serum concentrations of thyroid hormones and hepatic type I iodothyronine
5'-monodeiodinase (5'D-I) activity, an increase in the level of lipid
peroxidation was observed. The metal-induced decrease in hepatic 5'D-I
activity and serum triiodothyronine (T3) concentration was restored by
treatment with AA. However, AA could not restore the serum thyroxine
(T4) concentration. The increased level of LPO was also ameliorated by
AA. It appears that the protective effect of AA against cadmium-induced
thyroid dysfunction is mediated through its antioxidative action.
- The following study shows that vitamin C (ascorbic acid,
AA) can prevent the decrease in 5'D-I activity caused by cadmium and thereby
maintain T3 levels, but it can't restore T4 levels.
Role of testosterone in ameliorating the cadmium induced
inhibition of thyroid function in adult male mouse.
Gupta P; Kar A
Thyroid Research Unit, School of Life Sciences, Devi Ahilya University,
Vigyan Bhawan, Khandwa Road Campus, Indore 452 001, India.
Bull Environ Contam Toxicol, 58(3):422-8 1997 Mar
- The following study, while it has no abstract, indicates
that testosterone reduces the cadmium induced inhibition of thyroid
function. Other studies indicate that testosterone facilitates cadmium
excretion from the body. This is another indication that there are sex
differences in cadmium induced inhibition of thyroid function.
Evidence suggesting that cadmium induces a non-thyroidal
illness syndrome in the rat.
Pavia J´unior MA; Paier B; Noli MI; Hagm¨uller K; Zaninovich AA
CONICET, Hospital de Cl´inicas, University of Buenos Aires, Argentina.
J Endocrinol, 154(1):113-7 1997 Jul
The effect of in vivo administration of cadmium chloride on
the pituitary-thyroidal axis was assessed in 200 g body weight Wistar rats. A
dose of 2.5 mg/kg body weight was injected i.v. 24 h before the experiments
were initiated. Plasma thyroxine (T4) and tri-iodothyronine (T3)
concentrations in cadmium-treated rats were significantly (P < 0.01)
decreased, whereas plasma TSH failed to increase in response to low T4 and T3.
However, the TSH response to TRH and the pituitary content of TSH in these
rats were both normal. Cadmium induced a significant (P <
0.01) decrease in 4-h thyroidal 131I uptake and in thyroid/plasma
radioactivity ratio. The in vitro conversion of T4 to T3 in the pituitary
was significantly (P < 0.01) blocked by cadmium whereas there was no in
vivo effect. Parameters of peripheral T4 kinetics in cadmium-treated
rats, such as metabolic clearance rate (P < 0.01), fractional turnover rate
(P < 0.01), absolute disposal rate (P < 0.05), urinary clearance (P <
0.05) and faecal clearance (P < 0.05), were all decreased by cadmium.
The lack of response of TSH to low plasma T4 and T3 and the normal response
to exogenous TRH in this and in other non-thyroidal illness syndromes produced
by other pathologies suggest a decreased stimulation of pituitary thyrotrophs
by endogenous TRH.
- The significance of the following study is that in addition
to showing that cadmium decreases both T4 and T3, cadmium prevents TSH from
rising to correct the low T4 and T3 problem. This means that in our
era when doctors test only TSH to determine thyroidal function, many people
have undetected low thyroid function. This is a very important finding
since we see so many people with problems of low energy and weight gain, but
their doctors are telling them their thyroid function is normal. This
one study demonstrates the extreme importance of testing T4 and T3 and not
The following study might indicate that thyroid hormones are essential to
repair the damage caused by cadmium. This mechanism might explain why
ophthalmopathy increases in RAI treated humans.
Cadmium-induced acute lung injury: compromised repair response
Palmer KC; Mari F; Malian MS
Environ Res, 41(2):568-84 1986 Dec
The role of thyroid hormone in the pulmonary repair process following
cadmium chloride-induced acute injury, was assessed in the present study.
Thyroidectomized (Thyx), male Sprague-Dawley rats were exposed by
inhalation to cadmium chloride aerosol (CdCl2, 10 mg/m3).
Rats were sacrificed 1 hr after [3H]thymidine (3H-T) injection at
intervals up to 10 days after exposure. Thyroidectomy, followed by
CdCl2, produced earlier and more severe acute injury in the form of
alveolar hemorrhage edema and hyaline membrane formation, than CdCl2
alone. However, Type 2 cell hyperplasia was markedly reduced in this
group of rats compared with CdCl2 controls. Uptake of 3H-T by Thyx-CdCl2
lung tissue was only 40% of that measured in CdCl2 controls.
Autoradiographic studies indicated that Type 2 cell labeling was less
than 66% of controls up to 3 days after exposure. Cells obtained by lung
lavage of Thyx-CdCl2 rats were reduced in number up to 60% with respect
to controls, during the first week after exposure. Additionally, the
activities of lung antioxidant enzymes (glucose-6-phosphate
dehydrogenase, superoxide dismutase, and glutathione peroxidase were
significantly inhibited (45-55%) throughout the experiment in Thyx-CdCl2
animals compared with normal rats. In summary, thyroidectomy impairs the
repair response in CdCl2 lung damage by enhancing Type 2 cell damage,
reducing Type 2 cell proliferation, altering alveolar macrophage
populations, and depressing antioxidant defense systems.
The following study demonstrates the presence of
cadmium-binding proteins (CdBPs) present in humans in the kidney, liver,
pancreas, and thyroid gland, offering evidence that could be interpreted that
cadmium is an essential nutrient since the body has specific proteins to
- Cadmium-binding proteins in human
- Sato M; Takizawa Y
- Toxicol Lett, 11(3-4):269-73 1982 May
- Cadmium-binding proteins (CdBPs)
in the cytosol fractions from several organs of man orally exposed to cadmium
(Cd) were examined by gel chromatography. In kidney and liver most of the cadmium
(76-87%) in the cytosols was bound to metallothionein, and hepatic
metallothionein contained zinc also at a similar level. The pancreas cytosol
also contained a metallothionein-like CdBP, although its content was only
one-tenth of the hepatic one. In the thyroid gland a prominent CdBP, eluting
later than that of metallothionein, was observed; this CdBP was not detectable
in normal dogs, pigs, and oxen. No CdBPs were observed in other organs
including heart, muscle, genital organs, aorta, and bone. These results
suggest that CdBPs participate in accumulation and distribution of cadmium in
man, since organs containing CdBP such as kidney, liver, pancreas, and thyroid
gland show a tendency to accumulate high levels of cadmium.
- The following study shows that testosterone pretreatment
protects certain species, but not all species, from cadmium
toxicity. The method of protection is hypothesized to be increased
production of metallothionein, a protein that transports metals including
cadmium and zinc.
- Testosterone pretreatment mitigates cadmium toxicity
in male C57 mice but not in C3H mice.
- Shimada H; Bare RM; Hochadel JF; Waalkes MP
- Laboratory of Comparative Carcinogenesis, National Cancer
Institute, Frederick Cancer Research and Development Center, MD 21702-1201,
- Toxicology, 116(1-3):183-91 1997 Jan 15
- Previous work has indicated that testosterone
pretreatment protects against cadmium-induced toxicity in male rats while
other data indicate that pretreatment of mice with testosterone offers no
such protection against cadmium. Since cadmium toxicity may vary
widely with species and strain, we examined the effect of testosterone
pretreatment on cadmium toxicity in two strains of mice, one that is
sensitive (C3H) and one that is resistant (C57) to cadmium toxicity.
A single sc injection of 20 micromol CdCl2/kg to C3H mice or 45 micromol
CdCl2/kg to C57 mice proved very toxic, causing 50%, and 44% mortalities,
respectively. However, when C57 mice were pretreated with testosterone (5
mg/kg, s.c., at - 48, - 24, and 0 h) prior to cadmium (45 micromol/kg),
complete resistance to cadmium-induced lethality developed. Testosterone
had no effect on cadmium-induced lethality in C3H mice. Testosterone
prevented extensive hepatocellular damage caused by cadmium in C57
mice and also significantly reduced cadmium-induced elevations in
serum lactate dehydrogenase (LDH) activity and blood urea nitrogen (BUN),
which are indicators of hepatic and renal function, respectively. The
toxicokinetics of cadmium were apparently not affected by
testosterone pretreatment, as the distribution of cadmium to liver in
either strain was unchanged by the steroid. Cadmium-induced
metallothionein (MT) levels in liver and kidney of C57 mice were increased
in testosterone-pretreated mice given the higher doses of metal but no such
enhancement of MT synthesis occurred in C3H mice. This increase in MT may
provide some level of protection against cadmium toxicity in the C57 mice. These
results indicate that testosterone pretreatment prevents toxicity of cadmium
in male C57 mice, possibly through enhancement of MT synthesis, but has no
effect in male C3H mice.
Testosterone protects the body from cadmium toxicity.
This study shows that cadmium in turn decreases testosterone. Since
cadmium is a zinc antagonist and zinc is essential for the formation of
testosterone, cadmium may decrease testosterone production by zinc
This effect suggests interesting hypotheses about why
people smoke. Perhaps people smoke to reduce testosterone and become more
relaxed, since testosterone is a known activity stimulant. Women might
reduce their testosterone by smoking and "feel more feminine".
Just speculation, of course.
Unfortunately, taking up smoking has serious long-term
health consequences such as increased testicular cancer in men and increased
thyroid disease in women.
- The effects of continuous testosterone exposure on
spontaneous and cadmium-induced tumors in the male Fischer (F344/NCr)
rat: loss of testicular response.
- Waalkes MP; Rehm S; Devor DE
- Inorganic Carcinogenesis Section, Laboratory of Comparative
Carcinogenesis, Division of Basic Sciences, National Cancer Institute,
Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
- Toxicol Appl Pharmacol, 142(1):40-6 1997 Jan
- In the rodent testes, cadmium induces severe necrosis
followed by chronic degeneration. Cadmium is also an effective testicular
tumorigen, and a single dose produces a high incidence of Leydig cell
tumors. The mechanism of tumor formation is unknown, but pituitary
feedback, i.e., increased luteinizing hormone (LH) production due to low
circulating androgen, has been implicated in causation of proliferative
lesions within degenerate, hypofunctioning testes. Thus, the effects of
androgen replacement on the testicular toxicity of cadmium in Fischer
(F344/NCr) rats was studied. Groups (n = 50) of 10-week-old rats either
received testosterone implants that approximate normal circulating levels in
castrated rats or were left untreated. After 2 weeks of stabilization, rats
were given either 20 micromol CdCl2/kg, s.c., weekly for the next 5 weeks
(total dose 100 micromol/kg) or saline for a total of four treatment groups
(control, testosterone alone, testosterone + cadmium, or cadmium
alone). Portions of each group were killed either 10 weeks after initiation
of cadmium exposure (n = 10), for assessment of endocrine function,
or over the next 2 years (n = 40), for assessment of testicular neoplastic
lesions. At 10 weeks, cadmium reduced circulating testosterone in
nonimplanted rats by nearly 80% and induced a marked weight loss of the
testes (>70%) and sex accessory glands (reflected in a 50% reduction in
prostate mass). Testosterone implantation restored circulating
testosterone levels in cadmium-treated rats and prevented Cd-induced weight
loss of the sex accessory glands but not of the testes. Over 2 years, cadmium
alone induced a >84% incidence of Leydig cell neoplasia and a >97%
incidence of chronic degeneration, both significant increases over control
rates (60 and 0%, respectively). Testosterone implantation abolished both cadmium-induced
and spontaneously occurring Leydig cell tumors but had no effect on cadmium-induced
chronic testicular degeneration. Thus cadmium-induced hypofunction of the
testes, and subsequent loss of circulating testosterone, appears to be a
critical aspect in cadmium induction of tumors in the rat testes.
Sex differences in hepatic and renal cadmium
accumulation and metallothionein induction. Role of estradiol.
Blazka ME; Shaikh ZA
Department of Pharmacology and Toxicology, University of
Rhode Island, Kingston 02881.
Biochem Pharmacol, 41(5):775-80 1991 Mar 1
The role of estradiol in sex differences in hepatic and
renal cadmium accumulation and metallothionein (MT) induction was
investigated. Male and female rats and castrated males pretreated with
estradiol were injected either i.v. or s.c. with 10 mumol CdCl2/kg. Sex
differences in cadmium accumulation and MT induction were apparent
after s.c. but not i.v. administration. The female rats accumulated a
significantly greater concentration of cadmium in their liver than did the
males, as early as 1 hr after the s.c. injection. The elevated levels of cadmium
in the females were maintained for at least 10 days. Pretreatment of
castrated males with estradiol caused a similarly greater accumulation of
cadmium in the liver. Hepatic MT levels peaked in the females at 24 hr and
in males 48-72 hr after the cadmium injection and then declined to
lower levels. This superinduction of MT occurred only after the s.c.
administration of cadmium. MT levels in both sexes plateaued 5 days
after the s.c. injection to the levels that were similar to those seen in male
and female rats 24 hr after an i.v. injection. In animals injected s.c. with cadmium
the renal cadmium levels continued to rise for 5-10 days; however, in
animals injected i.v. the levels stabilized within 2 hr. The renal MT levels
in the females were significantly higher than in the males. Estradiol
pretreatment induced renal MT but did not affect renal cadmium accumulation. Thus,
the sex differences in hepatic cadmium accumulation and MT induction
are affected by the route and time after the administration of cadmium.
Furthermore, estradiol causes a more rapid uptake of cadmium by the liver
and also an enhanced induction of MT in both the liver and kidney.
- The following study demonstrates that estradiol (estrogen)
causes a more rapid uptake of cadmium by the liver and also an enhanced
induction of metallothionein (MT) in both the liver and kidney. This
is very good evidence that women are going to have greater cadmium
accumulation and this may be the mechanism by which women have a greater
incidence of thyroid disease than men. The question remaining is why
does estradiol increase cadmium accumulation? Is it just a by-product
of metallothionein production? Increased metallothionein production
would be beneficial for females because it increases the storage of trace
elements such as zinc which are needed for pregnancy. Perhaps the
problem is that our industrialized society has significantly increased
concentrations of cadmium. Then estradiol causes the inadvertent
accumulation of cadmium into female bodies because of this increased
concentration in our environment.
In contrast to the prior study, these researchers conclude
that estradiol increases the accumulation of cadmium without inducing the
synthesis of metallothionein. However, they agree that estradiol does
increase the accumulation of cadmium.
Stimulation of cadmium uptake by estradiol in the
kidney of male rats treated with cadmium.
Nishiyama S; Onosaka S; Taguchi T; Konishi Y; Tanaka K;
Department of Environmental and Occupational Health, Kochi
Medical School, Japan.
Biochem Pharmacol, 37(16):3091-6 1988 Aug 15
The present study was carried out to analyze the sex
differences in the retention of Cd in rats treated with a small amount of Cd,
and its mechanisms. Cd and Zn concentrations in the kidney and liver of female
rats treated with 28 nmol Cd or 1 nmole Zn were significantly higher than
those in male rats. Pretreatment with estradiol (1.8 mumol/kg of b.w., twice a
day, 6 consecutive days) increased the Cd and Zn concentrations in the kidney
of male rats treated with Cd or Zn. Incubation of MDCK cells with 10(-5) M
estradiol, 10(-5) M stilboestrol and 10(-5) M progesterone caused a
significant increase in Cd uptake. These results suggest that endogenous
female sex hormones may play a role in a higher concentration of Cd and Zn in
the kidney of female rats than that in male rats. The basal level of
metallothionein (MT) in the liver and kidney of control female rats was within
the same range as that in the control male rats. Cd and Zn accumulations
caused by pretreatment with estradiol in the kidney of male rats treated with
Cd or Zn were so low (Cd: 38 ppb, Zn: 1.0 ppb) as to be probably unable to
induce the synthesis of MT. An increase in the concentration of Cd in the
cultured renal cells occurred 1 hr after treatment with estradiol and Cd.
Pretreatment with estradiol alone also resulted in a modification of the
concentration of Na and K, which cannot be bound to MT. Together, all of
the above findings suggest that estradiol directly increases the accumulation
of Cd into the renal cells without inducing the synthesis of MT.
The following study shows that in a population of women,
cadmium concentrations increase with age and the cadmium/zinc ratio increases
with age indicating that zinc does not increase in proportion to cadmium.
This declining zinc/cadmium ratio could be an explanation for increased
incidence of hypothyroidism with age.
Cadmium and zinc concentrations in
Fiala J; Hrub´a D; R´ezl P
Institute of Preventive Medicine, Faculty of Medicine,
Masaryk University, Brno, Czech Republic.
Cent Eur J Public Health, 6(3):241-8 1998 Aug
Cadmium and zinc levels in
placentae of 688 women who delivered their children in two university hospitals
in Brno and in the regional hospital in Znojmo during January-June 1992 were
determined using AAS analytical method. Average value of zinc (54.6
micrograms/g) and cadmium (18.02 ng/g) concentrations found out in our
file are in accord with those ones reported in literature. Individual
differences in zinc contained in placentae occur uniformly. Very low
concentrations prevail for cadmium; values exceeding 100 ng/g of dry
basis are sporadic only. Zinc vs. cadmium concentrations values in
placenta are mutually positively correlated [correlation coefficient (factor) r
= +0.13, p < 0.001]. Cadmium content in placenta depends on mothers' age
and it is significantly higher in older women. No changes in zinc contained
in the placental tissue depending on mothers' age were found out. The mutual
ratio of zinc vs. cadmium content in a placental tissue is significantly
decreased in older mother (23.8 in older women, 41.2 in younger women, p <
The following study clearly indicates that cadmium is
associated with and probably causes bone demineralization, decreased bone
density in women, and decreased height in men. People living downwind of
zinc smelters face increased dangers because cadmium and zinc are found in the
same ore. Since all industrialized areas have increased amounts of air-,
water-, and food-borne cadmium, everyone is at risk for cadmium toxicity and
- Environmental exposure to cadmium, forearm bone
density, and risk of fractures: prospective population study. Public Health
and Environmental Exposure to Cadmium (PheeCad) Study Group.
- Staessen JA; Roels HA; Emelianov D; Kuznetsova T; Thijs L;
Vangronsveld J; Fagard R
- The Hypertensie en Cardiovasculaire Revalidatie Eenheid,
Departement Moleculair en Cardiovasculair Onderzoek, Katholieke Universiteit
Leuven, Belgium. firstname.lastname@example.org
- Lancet, 353(9159):1140-4 1999 Apr 3
- BACKGROUND: Chronic low-level exposure to cadmium may
promote calcium loss via urinary excretion. We undertook a prospective
population study to investigate whether environmental exposure to cadmium
lowers bone density and increases risk of fractures. METHODS: We
measured urinary cadmium excretion, a biomarker of lifetime exposure,
in people from ten districts of Belgium, of which six districts bordered on
three zinc smelters. We also measured cadmium in soil and in
vegetables from the districts, and collected data on incidence of fractures
and height loss. Bone density was measured at the forearm just above the
wrist by single photon absorptiometry, and calculated as the mean of six
proximal and four distal scans. FINDINGS: Mean cadmium excretion at
baseline was 8.7 nmol daily. Across the ten districts, mean cadmium
concentration in soil ranged from 0.8 to 14.7 mg/kg, and from 0.1 to 4.0
mg/kg dry weight in vegetables. Median follow-up was 6.6 years. Mean forearm
bone density in proximal and distal scans was 0.54 g/cm2 and 0.43 g/cm2 in
men, and 0.44 g/cm2 and 0.34 g/cm2 in women. In postmenopausal women, a
twofold increase in urinary cadmium correlated with 0.01 g/cm2 decrease in
bone density (p<0.02). The relative risks associated with doubled
urinary cadmium were 1.73 (95% CI 1.16-2.57; p=0.007) for fractures in women
and 1.60 (0.94-2.72, p=0.08) for height loss in men. Cadmium excretion
in districts near smelters was 22.8% higher (p=0.001) than in other
districts, with fracture rates of 16.0 and 10.3 cases per 1000 person-years,
respectively, and a population-attributable risk of 35.0%. INTERPRETATION:
Even at a low degree of environmental exposure, cadmium may promote skeletal
demineralisation, which may lead to increased bone fragility and raised risk
The following study supports the hypothesis that cadmium
toxicity effects are mediated by decreases in selenium and glutathione
peroxidase (a selenium-based antioxidant). Also noted, and this may be
very important, is that cadmium causes copper, zinc, and manganese to transfer
out of the mitochondria of cells. Other evidence suggests that a copper
deficiency in the mitochondria is a factor in the genesis of hyperthyroidism.
Title [Changes in trace elements
contents of renal cells in cadmium poisoning]
- Long M; Zhao J; Wang S
- Department of Preventive Medicine, Guiyang Medical College,
- Chung Hua Yu Fang I Hsueh Tsa Chih, 32(2):73-5 1998 Mar
- OBJECTIVE: To understand the possible role of trace
elements in renal damage caused by cadmium poisoning and its
mechanism. METHODS: An experimental animal model with renal damage caused by
cadmium poisoning was prepared, and trace elements contents in
subcellular components in renal cells, lipid peroxidation reaction, renal
function and its ultrastructural changes were determined. RESULTS: Uptake
of cadmium could cause transfer of copper, zinc and manganese mainly
distributed in the mitochondrion to cell nuclei and cytoplasm, and content
of selenium and activity of glutathione-peroxidase (GSH-px) in cytosol
declined and content of propandiolal increased. CONCLUSION: It
suggests that changes in trace elements contents, especially in selenium
content, during renal damage caused by cadmium poisoning, could correlate
with the increase of lipid peroxidation, and abnormal subcellular
distribution of trace elements was one of the important roles in renal
damage caused by cadmium poisoning.
The following study demonstrates that fasting or
interrupting the nutrient supply decreases the body's resistance to
Effects of fasting on cadmium toxicity, glutathione
metabolism, and metallothionein synthesis in rats.
Shimizu M; Morita S
Department of Environmental Health, Osaka City Institute of
Public Health and Environmental Sciences, Japan.
Toxicol Appl Pharmacol, 103(1):28-39 1990 Mar 15
Acute oral toxicity of Cd (as cadmium chloride) was
enhanced in rats fasted 24 hr, as shown by a markedly decreased LD50. To
examine the relationship among Cd toxicity, hepatic glutathione (GSH), and
metallothionein (MT) during fasting, rats were administered 75 mg Cd/kg orally
24 hr after fasting and euthanized after a further 4 or 24 hr for various
assays. Serum glutamic-pyruvic transaminase activity 24 hr after Cd treatment
was higher in fasted rats than in fed rats. Both total GSH and nonprotein
sulfhydryl (NPSH) concentrations in liver decreased to 50% of control levels
after 28 hr of fasting and returned to 75% of control values by 48 hr. Total
hepatic GSH concentration in fed rats decreased 4 and 24 hr after Cd
treatment, whereas that in fasted rats remained unchanged at 4 hr and
decreased significantly at 24 hr. Cd uptake by the liver (both concentration
and content) 24 hr after Cd treatment was higher in fasted rats than in fed
rats. Hepatic MT concentration was markedly increased by Cd treatment and
higher in fasted rats than in fed rats. There was no relationship between Cd
toxicity and hepatic thiobarbituric acid (TBA) value, an indicator of lipid
peroxidation. Fasting had no effect on hepatic GSH peroxidase and GSH
reductase activities. These enzymes probably are not involved in Cd toxicity.
On histological examination, focal degenerative and necrotic changes were
observed from the midlobular to the pericentral region in the livers of fed
rats 24 hr after Cd treatment. These changes were enhanced by fasting,
diffusing from the pericentral to the periportal region. Histochemical
examination revealed a heterogeneous distribution of GSH in the livers of fed
rats, with strong staining of GSH in the periportal region. This heterogeneous
distribution of GSH in liver was not observed in fed rats 4 hr after Cd
treatment or in fasted rats at 24 hr. The present results suggest that
hepatic GSH plays an important role in protection against Cd toxicity before
the onset of MT synthesis. Animals in bad condition, such as that resulting
from interruption of nutrient supply, cannot be protected against Cd toxicity
even if the hepatic MT level is high.
The following study indicates that vitamin C
(ascorbic acid) protects against cadmium toxicity without inducing
Effect of L-ascorbic acid pretreatment on cadmium
toxicity in the male Fischer (F344/NCr) rat.
Shiraishi N; Uno H; Waalkes MP
Inorganic Carcinogenesis Section, National Cancer Institute,
Frederick Cancer Research and Development Center, MD 21702-1201.
Toxicology, 85(2-3):85-100 1993 Dec 31
Some studies have indicated that cadmium-induced lethality
and selective injurious effects to specific tissues, such as testes or liver,
can be prevented by pretreatment with the antioxidant L-ascorbic acid
(ascorbic acid). However, the basis of this tolerance is unclear. We
examined the effects of ascorbic acid pretreatment on cadmium toxicity
in male Fischer (F344/NCr) rats. Cadmium treatment alone (25 mumol
CdCl2/kg, s.c.) proved lethal, causing a 93% mortality within 72 h, but in
rats pretreated with ascorbic acid (2 g/kg, s.c. 24, 12 and 1 h) cadmium-induced
lethality was nearly prevented. Hepatic lesions, including hepatocellular
necrosis, induced by cadmium were at least partially ameliorated by
ascorbic acid pretreatment. Ascorbic acid pretreatment had no effect on cadmium-induced
testicular lesions nor on cadmium content in testes, liver, kidney and
urine. Ascorbic acid alone modestly increased hepatic metallothionein (MT),
but not renal MT and had no effect on induction of hepatic or renal MT by cadmium.
In contrast to liver and kidney, testicular cadmium-binding protein (TCBP)
in rats exposed to cadmium alone decreased markedly. Moreover, the
level of TCBP decreased unexpectedly in ascorbic acid pretreated rats as
compared with control. These results indicate that ascorbic acid
pretreatment decreases the toxicity of cadmium in the rat without markedly
modifying its toxicokinetics or markedly stimulating MT synthesis.
The following two studies demonstrate the protective
effect of selenium against cadmium toxicity.
[The protective effect of simultaneous selenium
administration on acute cadmium toxicity and metallothionein]
Ohta H; Imamiya S; Yoshikawa H
Department of Health Administration, School of Hygenic
Sciences, Kitasato University.
Sangyo Igaku, 30(6):451-8 1988 Nov
The present study was conducted to elucidate the
protective action of simultaneous selenium administration against acute
cadmium toxicity. The remarkable testicular damages caused by cadmium,
that is, hemorrhagic inflammation, atrophy and necrosis, were lessened by
simultaneous selenium administration. Cadmium concentration in
blood, especially in plasma, increased significantly during the early period
after cadmium administration with selenium. Cadmium and selenium
in plasma were found in the same fractions of high molecular weight reported
by previous workers as the high molecular weight complex containing cadmium
and selenium. Cadmium in testis was also noted in the high molecular
weight fraction during the early period. However, cadmium in the high
molecular weight fraction of plasma and testis were unstable and decreased
rapidly by lapse in time. Cadmium concentration in liver was lower than
that in the group administered cadmium alone during the increasing
phase of plasma cadmium. However, in contrast with the decreased cadmium
level in plasma, cadmium in liver and testis increased gradually. Cadmium
increased in liver and testis were also found in the metallothionein fraction.
In the testis protected from acute cadmium toxicity, the inhibitory
effect of glutathione S-transferase activity by cadmium was not
detectable and the activity was maintained at the level of the control (saline
administered group). Moreover, the increased cadmium in the
metallothionein fraction was related to the decrease of cadmium in the
high molecular weight fraction of the testis homogenate. In addition, a
positive correlation was observed between metallothionein concentration and
glutathione S-transferase activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Graduate Institute of Medicine, Kaohsiung Medical University, Taiwan.
|Biometals 1999 Dec;12(4):353-9
Cadmium induced lipid peroxidation in rat testes and
protection by selenium.
Yiin SJ, Chern CL, Sheu JY, Lin TH
[Medline record in process]
The main goal of this study was to investigate the role of cadmium in the
promotion of lipid peroxidation in the homogenates of rat testes and the
effect of selenium on lipid peroxidation in testes of rats after cadmium
injection. Treatment of rats with cadmium resulted in a time- and dose-related
accumulation of the metal ions in testes. The concentrations of cadmium,
copper, zinc, selenium and iron in the tissues were determined by an atomic
absorption spectrophotometer and lipid peroxidation in testes was measured by
a spectrophotometer. Cadmium produced enhanced lipid peroxidation in testes.
These cadmium-induced changes were accompanied by a significant increase of
iron and copper, and a decrease of zinc in testes. Concurrent treatment with
selenium and cadmium reduced the cadmium-induced alterations in lipid
peroxidation and essential metal levels. Data suggest that lipid peroxidation
was associated with cadmium toxicity in testes and that the addition of
selenium was found to be effective in attenuation of this effect.
The following study demonstrates the protective effect of
zinc against the toxicity of cadmium in preembryos.
Zinc amelioration of cadmium toxicity on
preimplantation mouse zygotes in vitro.
Yu HS; Chan ST
Department of Zoology, University of Hong Kong.
Teratology, 37(1):13-9 1988 Jan
Zinc, at a concentration of 5 or 10 micrograms/ml medium,
has been shown to protect mouse preembryos in vitro from the toxicity of
cadmium at a concentration of 5 micrograms/ml medium after a simultaneous
treatment of the ions from four-cell to morula or from morula to blastocyst
for 24 hours. Such an amelioration indicates that cadmium toxicity is a
result of the unique property of the cadmium ion and is not due to an
alteration in the culture medium after the addition of an extra metallic ion.
Zinc probably ameliorates cadmium-treated mouse preembryo by competing
with cadmium for uptake or some other metabolic processes. In view of
the well-documented competition between cadmium and zinc ions for
binding sites in many other cell types, some macromolecules to which similar
divalent metallic ions bind competitively may also exist in the mouse
preembryo. This suggests that a protective mechanism dependent on the
metal-metal interactions begins to operate in the mouse preembryo at a very
early stage of differentiation before implantation.
Following is a summary the health problems caused by
cadmium and techniques for the removal of cadmium from the body.
Particularly important is the statement that cadmium has a half-life of over 10
years in the body.
- Cadmium therapeutic agents.
- Kelley C
- Department of Chemistry, Northern Arizona University,
Flagstaff, AZ 86011-5698, USA.Colleen.Kelley@nau.edu
- Curr Pharm Des, 5(4):229-40 1999 Apr
- Pollution of the environment with toxic metals has
increased dramatically since the beginning of the industrial revolution. Cadmium
is of particular concern because it accumulates in the human body with a
half-life exceeding 10 years and has been linked with a number of health
problems including renal tubular dysfunction, pulmonary emphysema,
significant kidney damage, and possibly osteoporosis. Moreover, in
1993 the International Agency for Research on Cancer (IARC) classified
cadmium and compounds containing cadmium as human carcinogens. The field
of cadmium intoxication therapy has seen increases in interest due to
its poignant toxicity in both humans and animals. Preliminary attempts to
combat acute cadmium poisoning included the use of the chelating agents
ethylenediaminetetraacetic acid (EDTA) and British anti-Lewisite (BAL). This
review will focus on the chemistry, biology, and effectiveness of cadmium
intoxication therapy to date. The toxicokinetics of cadmium mammals
will be discussed briefly to understand the extent and severity of
overexposure. An overview of cadmium chelation therapy will be given
with an emphasis on the measurable effectiveness of each and significant
structure activity relationships. Cadmium intoxication therapy will
be reviewed by their indicated routes of action: direct (chelation and
antagonism), indirect (induction), and symptom alleviation. The methods by
which cadmium therapeutics are evaluated (in vivo, in vitro) are to
be discussed. An evaluation of the clinical potential for promising
therapeutics will be given.
Here is a great study which shows that cadmium toxicity
decreases blood levels of magnesium. We see that in Graves' disease there
is evidence of cadmium toxicity and magnesium deficiency, manifested by
increased heart rate, heart arrhythmias, and tremors. The connection fits
with our observations.
Contribution to interaction between magnesium and toxic
metals: the effect of prolonged cadmium intoxication on magnesium
metabolism in rabbits.
Soldatovi´c D; Matovi´c V; Vujanovi´c D; Stojanovi´c Z
Department of Toxicological Chemistry, Faculty of Pharmacy,
University of Belgrade, Yugoslavia.
Magnes Res, 11(4):283-8 1998 Dec
The results obtained up to now indicate that increased intake
of some heavy metals causes disorder of bioelements metabolism leading to
their blood and organs decrease and higher elimination via the urine. As to
lead and magnesium, our investigations indicated that the reaction is
reversible and that increased intake of Mg eliminates Pb via urine. Some
other findings suggest similar relations between Mg and certain heavy metals,
thus pointing to significant role of Mg in toxicology of heavy metals and
announcing a new chapter in the toxicology of metals entitled 'Mg in
professional and ecotoxicology'. In this report we present the results of our
investigations on the effect of Cd on Mg metabolism. The experiment was
performed on rabbits given orally, every day, for 4 weeks 10 mg Cd/kg b.w. as
aqueous solution of CdCl2. Magnesium content was determined in blood, urine,
soft tissues and bones by the AAS method. Under the experimental
conditions, Cd lead to statistically significant decrease of blood Mg (p <
0.001 after day 16) which was associated with increased Mg elimination via
urine (p < 0.01). Statistically significant changes were not detected
in the tissues, except in the liver where we found enhanced Mg content (p <
0.05), while its level in the muscles decreased (p < 0.01).
As the following study indicates, cadmium has been
implicated in the formation of cataracts in chronic smokers. This study
shows that in addition to cadmium there is an accumulation of iron in the
lens. Vitamin E is shown to protect the lens by blocking iron accumulation
rather than blocking cadmium accumulation.
Cadmium and iron accumulation in
rat lens after cigarette smoke exposure and the effect of vitamin E (alpha-tocopherol)
Avunduk AM; Yardimci S; Avunduk MC; Kurnaz L
Karadeniz Technical University, School of Medicine,
Department of Ophthalmology, Trabzon, Turkey. email@example.com
Curr Eye Res, 18(6):403-7 1999 Jun
PURPOSE: Cadmium accumulation in the lens has been
implicated in cataractogenesis of chronic smokers. This study was planned
to evaluate whether or not in vivo cigarette smoke exposure causes cadmium
accumulation in rat lens, and possible protective effect and mechanism of
alpha-tocopherol (vitamin E) treatment on cataractogenesis. METHODS: 28
male Wistar rats were randomly divided into four equal groups. Group 3 and 4
rats were exposed to cigarette smoke over ninety consecutive days, and Group 1
and 2 rats were treated in a similar fashion but exposed only to room air.
Additionally, vitamin E was given to Group 2 and 4 rats. RESULTS:
Significantly higher iron levels were observed in the lenses of Group 3 rats
compared to other groups. With respect to cadmium, Group 3 and 4 rats had
significantly higher levels compared to Group 1 and 2 rats. Although vitamin E
treatment prevented iron accumulation in Group 4 rats, it had no effect on
cadmium concentrations. Distinct histopathological changes observed in
Group 3 rats were not present in Group 4 rats. CONCLUSION: Our study
demonstrates that in vivo cigarette smoke exposure causes accumulation of
cadmium in rat lens and IM vitamin E treatment does not affect this
accumulation. The protective effect of vitamin E treatment on smoke exposed
rat lens seems to be mediated by blockage of iron accumulation in the lens.
The following study shows that cadmium causes
decreases of selenium and calcium in the eye and increases of iron and copper in
the eye when selenium levels are low. Copper accumulation in the eye is
characteristic of certain copper accumulation diseases like Wilson's disease and
schizophrenia. Perhaps the copper accumulates there because of low
selenium and selenium supplementation might help those individuals' ability to
metabolize copper properly.
- Cadmium-induced alterations in
ocular trace elements. Influence of dietary selenium and copper.
- Jamall IS; Roque H
- Department of Health Services, Toxic Substances Control
Division, Technical Services, Sacramento, CA 94234-7320.
- Biol Trace Elem Res, 23():55-63 1989-90 Winter
- The present report demonstrates, for the first time, that
feeding rats 50 ppm cadmium for just 7 wk results in detectable
levels of cadmium in the eye of rats. Furthermore, these ocular cadmium
concentrations affect significant alterations in the levels of the essential
trace elements selenium, calcium, iron, and copper in the eye. Rats were fed
a low-selenium (less than 0.02 ppm selenium), high-copper basal diet (50 ppm
copper) supplemented with 0, 0.1, and 0.5 ppm selenium. The animals were
either untreated or treated with 50 ppm cadmium admixed with their
feed. Cadmium treatment resulted in significant reductions (up to 50%) in
ocular selenium. Furthermore, rats fed the basal diet and given 100 ppm cadmium
via their feed for 6 wk exhibited a 69% reduction in the activity of the
selenoenzyme, glutathione peroxidase, in the eye. Cadmium treatment also
resulted in reductions of up to 50% in ocular calcium, irrespective of
dietary selenium supplementation. Iron levels were increased by 30% in rats
fed the low-selenium diet and decreased by as much as 40% in rats fed the
selenium-supplemented diets, compared to animals fed identical levels of
selenium without cadmium. Ocular copper levels were significantly
increased only in rats fed the low-selenium diet and treated with cadmium.
Ocular zinc levels were not significantly affected by dietary cadmium
In the following two studies, a connection is shown between
cadmium toxicity and anosmia (loss of smell.) Brain accumulation of
injected cadmium was determined by using radioactive cadmium. Cadmium was
found to accumulate in the choroid plexus, pineal gland, area postrema,
trigeminal ganglia, and olfactory bulb. In the eye, cadmium accumulates in the
iris, ciliary body, and choroid. It is speculated that cadmium
accumulation in the olfactory bulb is related to the loss of ability to smell
(anosmia) seen in workers exposed to cadmium.
Interestingly, I have a friend who developed anosmia after
a fasting and cleansing diet high in green juices. He lives far from
pollution in the mountains and has never smoked tobacco. This may well be
good evidence that cadmium toxicity can result from excessive intake of green
leafy vegetables as well as from inhaling the smoke of another green leafy
vegetable, tobacco. As a possible testament to the long half-life of
cadmium, his anosmia lasted for two years.
Autoradiographic localization of cadmium in the rat
Neurotoxicology, 7(3):89-96 1986 Fall
Adult rats were injected intravenously with 109CdCl2 and the
distribution of the isotope within the brain and neighboring nervous
structures was subsequently studied by autoradiography. Cadmium
accumulated in regions outside the blood-brain barrier such as the choroid
plexus, pineal gland and area postrema, but did not appear in the brain
parenchyma. Uptake of cadmium was observed in the trigeminal ganglia
close to the nerve cells and in the olfactory bulbs. In addition, cadmium
accumulated in the iris, ciliary body and choroid of the eye, but not in the
optic nerves. The deposition of cadmium in the olfactory bulbs may be
related to the anosmia reported in workers exposed to this metal. The possible
harmful effects of accumulation of cadmium in restricted regions of the
brain and adjacent nervous structures are discussed.
Int J Occup Med Environ Health 1998;11(3):235-45
Olfactory disorders induced by cadmium exposure: a clinical study.
Rydzewski B, Sulkowski W, Miarzynska M
ENT Department, F. Raszeja Municipal Hospital, Poznan, Poland.
Cadmium, as a highly toxic metal, found widely in industry and in the
environment, is frequently included in the list of chemicals known to cause
olfactory impairment. The purpose of this study was to evaluate olfaction in
workers occupationally exposed to cadmium. The correlation between olfaction and
concentrations of cadmium in urine, blood and in the workplace air as well as
employment duration was examined in workers of the "CENTRA" S.A., an
electrochemical plant in Poznan. In this plant cadmium-nickel batteries are
produced, and there is chronic occupational exposure to cadmium in quantities
exceeding maximum allowable concentration (MAC). Of the 73 workers who completed
the evaluation, 53 people (72.7%) were smokers (10-40 cigarettes per day). The
examinations revealed numerous cases of hyposmia (26.0%) and parosmia (17.8%)
and one case of anosmia (1.4%). In the majority of people with olfactory
disorders, hypertrophic changes in the nasal mucosa, dependent on the duration
of employment, were identified. Statistically significant relationship between
olfactory impairment and cadmium concentration in blood, urine and the workplace
air was observed.
In the following study it is stated that, "Chronic
inhalation of cadmium at the workplace or in cigarette smoke is associated with
emphysema, a disease characterized by extensive disruption of lung connective
tissue." The study shows that cadmium disrupts the production of
collagen in the lungs by inhibiting fibroblast procollagen and proteoglycan
production. Since collagen production is dependent upon copper and cadmium
appears to be a direct antagonist of copper, copper depletion may be one of the
mechanisms by which cadmium disrupts collagen production.
Am J Respir Cell Mol Biol 1998 Sep;19(3):498-506
Title: Cadmium inhibits proteoglycan and procollagen
production by cultured human lung fibroblasts.
Chambers RC, Laurent GJ, Westergren-Thorsson G
Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, University
College Medical School, Rayne Institute, London, United Kingdom. R.Chambers@ucl.ac.uk
Chronic inhalation of cadmium at the workplace or in cigarette smoke is
associated with emphysema, a disease characterized by extensive disruption of
lung connective tissue. We have previously shown that cadmium, at
noncytotoxic doses, inhibits fibroblast procollagen production in vitro,
with maximal inhibitory effects of 69 +/- 6% (P < 0.01) at 30 µM
cadmium chloride (CdCl2). In this paper we show that at similar doses,
cadmium also inhibits proteoglycan synthesis, with values reduced by between
36 +/- 4% (P < 0.01) and 42 +/- 6% (P < 0.01) for proteoglycans secreted
into the culture media and associated with the cell layer, respectively. The
greatest inhibition was obtained for the major matrix-associated proteoglycans,
versican, decorin, and the large heparan sulfate proteoglycans, with synthesis
values reduced by between 60 and 70%. Biglycan and other heparan sulfate
proteoglycans were also affected, with synthesis values reduced by between 25
and 45%. In contrast, total protein synthesis was unaffected. Furthermore,
effects of cadmium at the protein level were mirrored by reduction in messenger
RNA levels for alpha1(I) procollagen, versican, and decorin. These data
support the hypothesis that cadmium may play an important role in the
pathogenesis of emphysema associated with chronic inhalation of cadmium fumes by
inhibiting the production of connective tissue proteins.
PMID: 9730878, UI: 98400991
The following study is very significant because it
explores the mechanisms of cadmium damage to lung cells and this may give us
clues about how cadmium damages the thyroid. It is shown that the cadmium
induced cell injury and lipid peroxidation are inhibited by catalase and
superoxide dismutase (copper, zinc based), two antioxidant enzymes.
Furthermore it was determined that hydrogen peroxide is the
main reactive oxygen species (ROS) involved. Hydrogen peroxide is the ROS
which stimulates thyroid hormone production in the thyroid, so this gives us a
direct connection from cadmium toxicity to increased thyroid hormone production
as we see in Graves' Disease.
Also it is shown that cadmium causes mitochondrial damage
by "significant dose-dependent changes of mitochondrial membrane
potential." This is very significant because we have been looking for a
mechanism by which the mitochondrial membrane's lithium-sodium counter transport
system is disrupted. This is the mechanism which appears to transport copper
into the mitochondria and may be the breakdown point where mitochondrial copper
deficiency is created, thereby inducing diseases such as hyperthyroidism and
manic depression. Remember that these are some long hypothetical leaps,
but this could provide a testable theory of the core mechanisms involved in both
hyperthyroidism and hypothyroidism. Cadmium may be the most significant
toxic substance in the etiology of thyroid disease.
Cadmium-induced oxidative cellular damage in human fetal
lung fibroblasts (MRC-5 cells).
Yang CF, Shen HM, Shen Y, Zhuang ZX, Ong CN
School of Public Health, Tongji Medical University, Wuhan, Peoples Republic
Epidemiological evidence suggests that cadmium (Cd) exposure causes
pulmonary damage such as emphysema and lung cancer. However, relatively
little is known about the mechanisms involved in Cd pulmonary toxicity. In the
present study, the effects of Cd exposure on human fetal lung fibroblasts (MRC-5
cells) were evaluated by determination of lipid peroxidation, intra-cellular
production of reactive oxygen species (ROS), and changes of mitochondrial
membrane potential. A time- and dose-dependent increase of both lactate
dehydrogenase leakage and malondialdehyde formation was observed in Cd-treated
cells. A close correlation between these two events suggests that lipid
peroxidation may be one of the main pathways causing its cytotoxicity. It was
also noted that Cd-induced cell injury and lipid peroxidation were inhibited by
catalase and superoxide dismutase, two antioxidant enzymes. By using the
fluorescent probe 2',7'-dichlorofluorescin diacetate, a significant increase of
ROS production in Cd-treated MRC-5 cells was detected. The inhibition of
dichlorofluorescein fluorescence by catalase, not superoxide dismutase, suggests
that hydrogen peroxide is the main ROS involved. Moreover, the
significant dose-dependent changes of mitochondrial membrane potential in Cd-treated
MRC-5 cells, demonstrated by increased fluorescence of rhodamine 123
examined using a laser-scanning confocal microscope, also indicate the
involvement of mitochondrial damage in Cd cytotoxicity. These findings
provide in vitro evidence that Cd causes oxidative cellular damage in human
fetal lung fibroblasts, which may be closely associated with the pulmonary
toxicity of Cd.
PMID: 9294717, UI: 97440499
- The following review states that
|J Environ Pathol Toxicol Oncol 2001;20(2):77-88
Oxidative mechanisms in the toxicity of chromium and
Stohs SJ, Bagchi D, Hassoun E, Bagchi M.
Department of Pharmaceutical and Administrative Sciences, Creighton
University School of Pharmacy and Allied Health Professions, Omaha, NE
Chromium and cadmium are widely used industrial chemicals. The toxicities
associated with both metal ions are well known. However, less information is
available concerning the mechanisms of toxicity. The results of in vitro and
in vivo studies demonstrate that both cations induce an oxidative stress
that results in oxidative deterioration of biological macromolecules.
However, different mechanisms are involved in the production of oxidative
stress by chromium and cadmium. Chromium undergoes redox cycling, while cadmium
depletes glutathione and protein-bound sulfhydryl groups, resulting in
enhanced production of reactive oxygen species such as superoxide ion,
hydroxyl radicals, and hydrogen peroxide. These reactive oxygen species
result in increased lipid peroxidation, enhanced excretion of urinary lipid
metabolites, modulation of intracellular oxidized states, DNA damage,
membrane damage, altered gene expression, and apoptosis. Enhanced production
of nuclear factor-kappaB and activation of protein kinase C occur.
Furthermore, the p53 tumor suppressor gene is involved in the cascade of
events associated with the toxicities of these cations. In summary, the
results clearly indicate that although different mechanisms lead to the
production of reactive oxygen species by chromium and cadmium, similar
subsequent mechanisms and types of oxidative tissue damage are involved in
the overall toxicities.
The following study shows that cells protect
themselves from cadmium toxicity by production metallothionein (MT), which is a
thiol-rich protein (contains sulfur). We have seen that cadmium induces
production of hydrogen peroxide and this chemical releases free radicals which
increase thyroid hormone production. Now here is evidence that sulfur
groups called thiols bind with cadmium to protect cells from cadmium induced
damage. Antithyroid drugs such as Tapazole and PTU contain these thiols
Toxicology 1995 Apr 12;98(1-3):1-13
Title: Oxidant resistance of cadmium-adapted human lung
Hart BA, Prabhu RM, Eneman JD, Durieux-Lu CC, Janssen AM,
University of Vermont College of Medicine, Department of Biochemistry,
Burlington 05405, USA.
Metallothionein (MT) is a metal and thiol-rich protein readily induced by
cadmium (Cd) exposure. In vitro experiments have demonstrated that MT is
able to serve as a scavenger of hydroxyl radicals as well as superoxide anions,
albeit to a lesser extent. The role of MT as a mediator in Cd induced oxidant
resistance was investigated in a nontransformed human lung fibroblast cell line
(IMR-90). Cells were passaged three times either in a Cd-containing medium (8.9
microM CdCl2) or in a medium which lacked Cd. Cellular MT content, as
quantitated by a modification of the heme/109Cd binding assay, increased
significantly with each passage in Cd. Immunocytochemistry studies revealed that
all Cd-pretreated cells contained MT and that MT was localized in both
cytoplasmic and nuclear compartments. Immunolabeling was more intense in some
cells compared to others. Very slight immunolabeling was observed in
physiological control cells, grown in the absence of Cd, and virtually no
staining was observed in Cd-adapted or non-adapted cells when primary antibody
was omitted. Using the xanthine/xanthine oxidase system as a generating system
for active oxygen species, we found that the magnitude of cell injury for Cd-adapted
and non-adapted fibroblasts was dependent upon oxidant concentration and
duration of oxidant exposure. Cd-adapted fibroblasts, which were
characterized by over-expression of MT, were significantly more resistant to
injury by active oxygen species and also exhibited a greater ability to scavenge
extracellular hydrogen peroxide compared to cells with no previous history of Cd
exposure. Experiments with aminotriazole demonstrated that catalase was not
a major contributor to the additional hydrogen peroxide scavenging capacity of
Cd-adapted cells. The data presented in this report are consistent with
involvement of MT in protecting critical cellular targets from reactive oxygen
PMID: 7740538, UI: 95259056
The following study shows that both zinc and
selenium are key mineral antagonists to cadmium and protect cells from
cadmium-induced toxicity. There is also a list of chemicals which protect
cells from cadmium toxicity which includes dimethyl sulfoxide (DMSO).
Ethanol was shown to not protect cells from cadmium toxicity and other studies
have shown that ethanol increases cadmium accumulation into the body.
Cell Biol Toxicol 1994 Jun;10(3):191-205
Title: Antagonism of cadmium cytotoxicity by
Department of Chemistry, Adelphi University, Garden City, New York 11530.
Studies on the antagonism of toxicity can provide information about toxic
mechanisms and suggest chemotherapeutic strategies. A rapid cell growth assay
that measures the effects of test agents on the accumulation of cell protein (Shopsis
and Eng, Toxicol. Lett. 1985;26:1) has been applied to studies of the antagonism
of the cytotoxicity of cadmium. Exposure of Balb/c mouse 3T3 cells to 15 mumol/L
Cd2+ for 24 h or 7 mumol/L Cd2+ for 48 h caused a 50% decrease in total cell
protein. Zn2+ and selenite ion, antagonists of Cd toxicity in vivo,
antagonized Cd2+ cytotoxicity when added in micromolar concentrations at the
initiation of exposure to Cd2+. A diverse group of chemicals that can induce
differentiation in vitro in cultured erythroleukemia and other cells were also
found to antagonize the cytotoxic effects of Cd2+ to 3T3 cells. Dimethyl
sulfoxide (DMSO), hexamethylene bisacetamide, N,N-dimethyl formamide, N-methyl
formamide, dimethyl acetamide, hypoxanthine, hemin, ouabain, and sodium
butyrate, when added to cultures simultaneously with Cd2+, each antagonized Cd2+
toxicity. These agents were used at concentrations equal to or lower than
the concentrations at which they induce cellular differentiation. Other
cytotoxicity assays and morphological studies confirmed these observations. DMSO
added as much as 6 h after the initiation of a 24-h exposure to Cd2+ still
protected cells; conversely, pretreatment of cultures with butyrate or DMSO
for 24 h followed by their removal did not confer protection against subsequent
Cd2+ challenge. Ethanol and methanol (noninducers of differentiation) did not
antagonize Cd2+ cytotoxicity, and differentiation-inducing agents did not
protect the cells from Zn(2+)- or Hg(2+)-induced cytotoxicity. DMSO treatment
does not induce an increase in the concentrations of metallothionein or
glutathione in these cells.
In the following study the authors speculate that
cadmium toxicity is the result of cadmium's affinity for sulfhydryls and
the cell protects itself from cadmium toxicity by increasing production of
metallothionein, glutathione peroxidase (selenium compound), and
Toxicol Appl Pharmacol 1994 May;126(1):114-23
Title: Alterations in cytoskeletal organization and
homeostasis of cellular thiols in cadmium-resistant cells.
Li W, Kagan HM, Chou IN
Department of Biochemistry, Boston University School of Medicine,
To understand further the mechanisms of cadmium toxicity, cytoskeletal
organization and homeostasis of cellular thiols were examined in
cadmium-resistant cells isolated from Swiss mouse 3T3 cells by incubation in
graded concentrations of CdCl2 (Cd2+) in the culture medium. Cd(2+)-resistant
cells displayed profound alterations in their cytoskeletal organization
characterized by the appearance of many elongated, tadpole-shaped cells with a
high density of microtubules (MT) and microfilaments (MF), with the former being
mainly distributed along the long axis of the cell. Exposure of Cd(2+)-resistant
cells to 50 microM Cd2+ for 16 hr did not cause apparent cytoskeletal
perturbations, whereas treatment of parental cells with 5 microM Cd2+ for the
same duration produced a severe loss of MT and smeared patches of MF. Thus, the
cytoskeleton of Cd(2+)-resistant cells is markedly more preserved and protected
against Cd2+ damage than that of their parental counterparts.
Cd(2+)-resistant cells contained a higher basal level of protein sulfhydryls (PSH)
in both the cytoskeletal and cytosolic fractions than the parental cells.
Exposure to 50 microM Cd2+ further increased cellular PSH contents, reaching 192
and 215% of the basal levels for the cytoskeletal and cytosolic fractions,
respectively. Although 5 microM Cd2+ exposure also elevated the amounts of PSH
in parental cells, the "absolute" values were still below the
corresponding basal levels in Cd(2+)-resistant cells. Furthermore,
Cd(2+)-resistant cells also exhibited enhanced basal levels of metallothionein
and cellular glutathione (GSH), amounting to 19- and 2.1-fold of the
parental basal levels, respectively. Thus, the Cd(2+)-resistant cells
produced larger quantities of both protein and nonprotein thiol-containing
elements than the parental cells. Interestingly, exposure of
Cd(2+)-resistant cells to 50 microM Cd2+ also further increased metallothionein
and cellular GSH to 178 and 138% of the basal levels, respectively. Based on
the affinity of Cd2+ for sulfhydryls as a mechanism of Cd2+ toxicity, we propose
that the coordinately increased levels of metallothionein, GSH, and PSH in
Cd(2+)-resistant cells would provide a mechanistic basis for the homeostasis of
cellular thiols which may collectively contribute to the cytoskeletal
preservation by protecting the cytoskeleton from Cd2+ insult.
The following study shows that transforming growth factor
beta (TGF beta 1) induces a tolerance to cadmium in
cultured endothelial cells.
Toxicology 1994 Mar 11;88(1-3):69-79
Transforming growth factor beta-induced tolerance to
cadmium cytotoxicity in cultured vascular endothelial cells.
Kaji T, Ohkawara S, Yamamoto C, Sakamoto M, Kozuka H
Department of Environmental Science, Faculty of Pharmaceutical Sciences,
Hokuriku University, Kanazawa, Japan.
We investigated whether or not transforming growth factor beta (TGF beta
1) affects the sensitivity to cadmium of bovine aortic endothelial cell in a
culture system. Cadmium cytotoxicity was evaluated by [3H]adenine release assay.
It was found that pretreatment with recombinant human TGF beta 1 (rhTGF beta 1)
of the confluent cultures resulted in a reduction of cadmium cytotoxicity,
suggesting that the cytokine induced a tolerance to cadmium in the cells. Such a
tolerance was induced slightly by either recombinant human tumor necrosis factor
alpha or recombinant human basic fibroblast growth factor but not by recombinant
human interleukin-1 beta and -6; rhTGF beta 1 was the most potent inducer. rhTGF
beta 1 failed to induce the tolerance in the presence of anti-rhTGF beta 1
antibody. Pretreatment with the antibody alone caused a significantly sensitive
response to cadmium, suggesting that endogenous TGF beta 1 can physiologically
contribute to protection against cadmium cytotoxicity in endothelial cells. The
accumulation of cadmium was increased in the extracellular fraction but
significantly decreased in the intracellular fraction of the cells by
pretreatment with rhTGF beta 1. The cadmium content was significantly decreased
in the particulate fraction but not in the cytosol fraction. Gel filtration
chromatography of the cytosol fraction revealed that cadmium was bound to
high-molecular-weight protein and metallothionein; both peaks were slightly
increased by pretreatment with rhTGF beta 1. It was concluded that rhTGF beta
1 induces a tolerance to cadmium in cultured endothelial cells, caused by a
decrease in the cadmium accumulation in the particulate fraction of the cells.
TGF beta 1 may serve as a protective factor against cadmium cytotoxicity in
vascular endothelial cells.
The following study shows that older cells are more
sensitive to the toxic effects of cadmium because of reduced ability to produce
Exp Gerontol 1993 Jan-Feb;28(1):17-38
Metallothionein expression and stress responses in aging
human diploid fibroblasts.
Luce MC, Schyberg JP, Bunn CL
Department of Biology, University of South Carolina, Columbia 29208.
Metallothioneins (MTs) are low molecular weight proteins with a high
cysteine content that are inducible by heavy metals and by other conditions of
environmental stress. This laboratory was investigated in human diploid
fibroblasts the induction of MTs by cadmium and by dexamethasone, and the
induction of heat shock proteins, as models for age-related changes in gene
expression that reflect the ability of old cells to respond to environmental
stress. Old cells were more sensitive to the toxic effects of CdCl2 in the
concentration range 100-175 microM. Analysis of 35S-cysteine-labelled cell
extracts by polyacrylamide gel electrophoresis and fluorography showed that in
the absence of any inducer, old cells have a 3.7-fold increase over young cells
in the basal level of MT. The rate and extent of induction of MT by CdCl2 was
reduced in old cells: Exposure of old cells to 100 microM CdCl2 for 18 h
resulted in MT levels about 33% of the amount in young cells. Northern blot
analysis showed that the changes in MT protein levels occurred in parallel with
changes in mRNA levels, which implicates transcriptional control as the origin
of these aging changes. These young/old differences in MT synthesis were
maintained in density-arrested cultures, indicating that the aging changes were
not due to differences in the cell cycle status of these cell populations. The
rate and extent of induction of a 68-kDa heat shock protein were also reduced in
old cells, which showed an increase in basal, uninduced level of this protein
similar to MT. In contrast, old cells retained the ability to synthesize MTs in
response to dexamethasone at a rate similar to that in young cells.
The following study elaborates how cells develop
Life Sci 1999;65(14):PL177-82
Reduced uptake and enhanced release of cadmium in
cadmium-resistant metallothionein null fibroblasts.
Yanagiya T, Imura N, Kondo Y, Himeno S
Department of Public Health and Molecular Toxicology, School of
Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Metallothionein (MT) is known to play a predominant role in the protection of
cells from cadmium (Cd) toxicity. To investigate other factors involved in Cd
resistance, we established Cd-resistant cell lines from simian virus
40-transformed MT null fibroblasts. Cd-resistant MT null cells, Cd-rA7 and
Cd-rB5, developed approximately 10-fold resistance to Cd compared to parental
cells, but showed no cross-resistance to Zn, Cu, Hg, Ni, As, cisplatin or H2O2.
Accumulation of Cd in the resistant cells was 13-18% of that of parental cells
after treatment with Cd for 24 h. A short-term experiment revealed that the rate
of Cd incorporation into the Cd-resistant cells was suppressed, and the rate of
Cd release was enhanced in the resistant cells compared with that of parental
cells. These results indicate that the altered transport of Cd, slow uptake
and rapid release, may confer resistance to Cd on the Cd-resistant cells
established from MT null fibroblasts.
PMID: 10530804, UI: 99458387
The following study should be a concern to anyone
reading this on a computer. Three novel compounds which are used in the
manufacture of computers were tested for toxicity. These compounds may be
inhaled by workers in the semiconductor industry or users of computers.
While I believe that users of computers would have very little exposure, it
would be good to find out how much.
The most toxic of the three tested was found to be cadmium
telluride and presumably the toxicity is due to the cadmium. I consider it
another example of how cadmium from industrial uses can get into our bodies and
probably cause thyroid disruption.
Comparative pulmonary absorption, distribution, and
toxicity of copper gallium diselenide, copper indium diselenide, and cadmium
telluride in Sprague-Dawley rats.
Morgan DL, Shines CJ, Jeter SP, Blazka ME, Elwell MR, Wilson
RE, Ward SM, Price HC, Moskowitz PD
National Institute of Environmental Health Sciences, Research Triangle Park,
North Carolina 27709, USA.
Copper gallium diselenide (CGS), copper indium diselenide (CIS), and cadmium
telluride (CdTe) are novel compounds used in the photovoltaic and semiconductor
industries. This study was conducted to characterize the relative toxicities of
these compounds and to evaluate the pulmonary absorption and distribution after
intratracheal instillation. Female Sprague-Dawley rats were administered a
single equimolar dose (70 mM) of CGS (21 mg/kg), CIS (24 mg/kg), CdTe (17
mg/kg), or saline by intratracheal instillation. Bronchoalveolar lavage fluid (BALF)
protein, fibronectin, inflammatory cells, lung hydroxyproline, and tissue
distribution were measured 1, 3, 7, 14, and 28 days after instillation. Relative
lung weights were significantly increased in CIS- and CdTe-treated rats at most
time points. Inflammatory lesions in the lungs consisting of an influx of
macrophages, lymphocytes, and PMNs were most severe in CdTe-treated rats,
intermediate in CIS-treated rats, and minimal in rats receiving CGS. Hyperplasia
of alveolar type 2 cells was present in CIS- and CdTe-treated rats and was
greatest in CdTe-treated rats. Pulmonary interstitial fibrosis was observed in
CdTe-treated rats at all time points. All three compounds caused marked
increases in total BALF cell numbers, with the greatest increase observed in
CIS-treated rats. BALF protein, fibronectin, and lung hydroxyproline were
significantly increased in all treated animals and were highest in CdTe-treated
animals. There was no apparent pulmonary absorption or tissue distribution of
CGS. Indium levels increased in extrapulmonary tissues of CIS-treated rats,
although Cu and Se levels remained unchanged. CdTe was absorbed from the lung
to a greater extent than CGS and CIS. Cd and Te levels decreased in the lung
and increased in extrapulmonary tissues. Of these compounds CdTe presents the
greatest potential health risk because it causes severe pulmonary inflammation
and fibrosis and because it is readily absorbed from the lung may potentially
cause extrapulmonary toxicity.
The following study helps give us an idea about how
toxic cadmium is in relation to other metals with known or presumed biological
function. Cadmium is three times more toxic than arsenic. However,
if it could be shown that cadmium is an essential nutrient, as I suspect all of
the other metals mentioned are, then it also can give us an idea about how much
we might need of it in relation to the other minerals.
J Toxicol Environ Health 1990 May;30(1):23-31
Teratogenicity of metals to chick embryos.
Gilani SH, Alibhai Y
Department of Anatomy, New Jersey Medical School, Newark 07103.
The present study examines the effects of heavy metals on chick embryogenesis.
The metals included were cadmium, arsenic, cobalt, copper, indium, iron,
manganese, and molybdenum. Salts of each of the metals were dissolved in
saline and injected into the air sacs on d 2 of incubation. Dose levels varied
with the metal to be tested. Control eggs were injected with an equivalent
volume of saline (0.1 ml/egg). On d 14, the live embryos were removed from the
eggs and examined for gross malformations. From the LD50 values, the toxicity
relationship between the metals is cadmium greater than arsenic greater than
cobalt greater than copper greater than indium greater than molybdenum greater
than manganese greater than iron. The LD50 values were 3, 9, 38, 58, 121, 333,
765, and 1185 micrograms/egg, respectively. The gross malformations observed
were reduced body size, micromelia, twisted neck, hemorrhage, everted viscera,
and microphthalmia. Arsenic and cobalt were observed to be more teratogenic than
other metals. This study showed that the metals tested were both toxic and
teratogenic to varying degrees in chick embryogenesis.
The following study shows that as cadmium blood
levels increase, TSH increases and FT4 decreases. Another study with
implications that cadmium causes thyroid disease.
Environ Health Perspect 1999 Oct;107(10):843-9
Exposure to polychlorinated biphenyls and levels of
thyroid hormones in children.
Osius N, Karmaus W, Kruse H, Witten J
NORDIG Institute for Health Research and Prevention, Hamburg, Germany. NOsius@t-online.de
As part of an epidemiologic study on exposure to a toxic waste incineration
plant we investigated whether blood concentrations of polychlorinated biphenyls
(PCBs), lead, and cadmium, as well as concentration of mercury in 24-hr urine
samples were associated with thyroid hormone status. As an indication of status,
we determined levels of thyroid-stimulating hormone (TSH), free thyroxine
(FT(4)), and free triiodothyronine (FT(3)) in children living in households
where [less than/equal to] 10 cigarettes were smoked per day. Eight PCB
congeners (PCBs 101, 118, 138, 153, 170, 180, 183, and 187) were measured in
whole blood samples. Of these, seven congeners (PCB 101 was not detected in any
sample) and the sum of all PCB congeners were analyzed as predictors for thyroid
hormone status in separate linear regression models adjusted for potential
confounders. In addition, the possible effects of cadmium, lead, and mercury on
levels of thyroid hormones were examined. Blood concentrations and information
on questionnaire data were available for 320 children 7-10 years of age. We
found a statistically significant positive association between the mono-ortho
congener PCB 118 and TSH as well as statistically significant negative
relationships of PCBs 138, 153, 180, 183, and 187 to FT(3). There was no
association for the PCB congeners and FT(4). Blood cadmium concentration was
associated with increasing TSH and diminishing FT(4). Blood lead and urine
concentration of mercury were of no importance to thyroid hormone levels. The
results stress the need for future studies on the possible influences of PCB and
cadmium exposure on thyroid hormones, particularly in children. These studies
should also take neurologic development into account.
The following study indicates that DMPA, but not DMSA or
DMPS, may be an effective chelator for cadmium removal from the body.
Fundam Appl Toxicol 1990 Apr;14(3):598-607
Determination and metabolism of dithiol chelating agents.
VII. Biliary excretion of dithiols and their interactions with cadmium and
Zheng W, Maiorino RM, Brendel K, Aposhian HV
Department of Pharmacology and Toxicology, University of Arizona, Tucson
N-(2,3-Dimercaptopropyl) phthalamidic acid (DMPA), meso-dimercaptosuccinic
acid (DMSA), and 2,3-dimercapto-1-propanesulfonic acid (DMPS) are dithiol
chelating agents with antidotal activity for lead, mercury, arsenic, and other
heavy metals. The biliary excretion of these compounds was studied in male
Sprague-Dawley rats. After iv administration of DMPA, 72% of the injected dose
was recovered in the bile. Half of the recovered DMPA was in the unaltered form
(parent compound) and the other half was in the altered form (parent compound
recovered after chemical reduction by DTT). An altered, presumably disulfide,
form of DMPS was found in the bile. Neither unaltered nor altered DMSA was
detected in the bile. DMPA (0.10 mmol/kg), given to rats 3 days after exposure
to Cd, elicited within 30 min a 20-fold increase in biliary Cd excretion. The
increase of biliary Cd by DMPA was dose-related and not due to an increase of
bile flow rate. DMSA and DMPS did not significantly affect the biliary excretion
of Cd. Incubation of DMPA or DMSA with Cd-saturated metallothionein (MT)
resulted in the removal of Cd from MT. DMPA was more active than DMSA in this
respect. The evidence strongly supports the mechanism that the increase of
biliary cadmium following DMPA administration is the result of DMPA entering
cells and mobilizing and removing the cadmium from MT. The removal of cadmium
from metallothionein by dithiol chelating agents provides another dimension to
their mechanisms of action and may provide an important new tool for the study
of cadmium as well as metallothionein.
The following study indicates that BAL is an
effective chelator of cadmium.
J Toxicol Environ Health 1980 Mar;6(2):393-401
Biliary excretion of cadmium in rat. VI. Mobilization of
cadmium from metallothionein by 2,3-dimercaptopropanol.
Cadmium was preferentially bound to metallothionein in tissues 24 h after
CdCl2 injection. Of a number of chelating agents examined, only
2,3-dimercapto-1-propanol (BAL) was effective in mobilizing Cd from
metallothionein into bile. Structurally similar dithiols such as
1,3-dimercaptopropanol and 2,3-dimercapto-1-propanesulfonic acid were not
effective. Diethylenetriamine pentaacetic acid increased only the urinary
excretion of Cd. Biliary excretion of Cd increased with increasing dose of
BAL, and there was a concurrent decrease in hepatic Cd levels without any
increase in renal concentration. BAL was effective even 14 d after Cd
injection. The form of Cd excreted in the bile after BAL injection in chronic
exposure has not yet been characterized. Initial studies suggested that it was
not metallothionein but was a low-molecular-weight Cd complex, probably with
The following study demonstrates the effectiveness
of using alpha lipoic acid (a supplement available in a health food store and
which I have used) in protecting the liver from cadmium toxicity.
Jpn J Med Sci Biol 1996 Apr;49(2):39-48
Relationship between glutathione and DL alpha-lipoic acid
against cadmium-induced hepatotoxicity.
Sumathi R, Baskaran G, Varalakshmi P
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic
Medical Sciences, University of Madras, India.
Cadmium, a divalent metal toxicant, preferentially localizes in hepatocytes
and causes liver injury. DL alpha-lipoic acid is a dithiol which is effective
in rendering protection against cadmium-associated liver damage, by virtue of
its two sulfhydryl moieties. Lipoate was administered to cadmium-exposed
rats which were either prior administered with buthionine sulfoximine to deplete
liver glutathione or not. During lipoate treatment, significant protection was
rendered against cadmium toxicity even under glutathione-depleted experimental
condition. This highlights the antioxidant property of lipoic acid and its
efficacy in mitigating cadmium-associated liver assault even in the absence of
FOOD SOURCES OF CADMIUM
The following study is important because it states
that cadmium absorption is increased by co-administration of milk and in
conjunction with iron deficiency. The author also states that mercury
accumulation is increased by a factor of ten in suckling pigs and that milk
increases the bioavailability of mercury. If the absorption of cadmium and
mercury are increased significantly by milk, this is interesting because of the
fact that the factor in milk that might cause this is estrogen.
We have seen that females accumulate cadmium and mercury at
a much higher rate than males and it seems that estrogen is an accelerator of
heavy metal (and maybe all metal) accumulation. Milk may also increase
heavy metal accumulation by providing additional estrogen. It could be
other factors in milk, but this hypothesis needs to be investigated.
The author states that cadmium toxicity can cause
microcytic hypochromic anemia, a type of anemia where the red blood cells are
both smaller and have less hemoglobin. We have seen that anemia is highly
associated with thyroid disease.
|Z Ernahrungswiss 1990 Mar;29(1):54-73
Title: The toxicological estimation of the heavy metal content (Cd, Hg,
food for infants and small children
[Article in German]
Walther-Straub-Institut fur Pharmakologie und Toxikologie der
Ludwig-Maximilians-Universitat, Munchen, FRG.
There are differences between young and adult organisms regarding
toxokinetic aspects and clinical manifestations of heavy metal
intoxications. Chronically, toxic Cd intake causes a microcytotic
hypochromic anemia in young rats at lower exposure levels and after shorter
exposure periods than in adult animals. Cd absorption is increased by
co-administration of milk and in conjunction with iron deficiency. After
long exposure periods toxic Cd concentrations accumulate in the kidney
cortex; this process starts very early in life. In 3-year-old children Cd
concentrations in the kidney can reach up to one-third of those found in
adults. Hg++ and methyl-Hg can cause Hg encephalopathia, and frequently
cause mental retardation in adults. Correspondingly, Hg++ accumulation in
the brains of suckling rats is approx. 10 times higher than in grown
animals. Milk increases the bioavailability of Hg++. In suckling rats Hg is
bound to a greater extent to ligands in the erythrocytes. Methyl-Hg
concentrations in breast milk reach 5% of those in maternal plasma and that
is a severe hazard for breastfed children of exposed mothers. Toxic Pb
concentrations can lead to Pb encephalopathia. A high percentage of
surviving children have seizures and show signs of mental retardation.
Anemia and reduced intelligence scores were recently observed in children
after exposure to very low levels of Pb. Pb absorption is increased in
children and after co-administration of milk. There are no definite proofs
for carcinogenesis or mutagenesis after oral exposure to Cd, Hg, and Pb in
man. Heavy metal concentrations were found in the same order of magnitude in
commercial infant formulas and in breast milk. When infant formulas are
reconstituted with contaminated tap water, however, Pb and Cd concentrations
can be much higher. The average heavy metal uptake from such diets exceeds
the provisional tolerable weekly intake levels set by the WHO for adults,
calculated on the basis of an average food intake and a downscaled body
weight. These considerations do not even provide for differences in
absorption and distribution or for the increased sensitivity of children to
heavy metal exposure. However, dilution effects for essential heavy metals
were observed in fast-growing young children; this effect might be
extrapolated to toxic metals. These theoretical considerations are compared
with epidemiological evidence. A health statistic from Baltimore shows a
decline of Pb intoxications in infants. This observation correlates with a
simultaneous decline in exposure to Pb which was due, for example, to
decreased use of lead dyes in house paints and the abolition of tin cans for
The following study indicates that wheat bran is preferable
to sugar-beet fiber and carrots to limit gastrointestinal absorption of cadmium.
Br J Nutr 1998 Aug;80(2):205-11
Accumulation of cadmium from wheat bran, sugar-beet fibre,
carrots and cadmium chloride in the liver and kidneys of mice.
Lind Y, Engman J, Jorhem L, Glynn AW
Swedish National Food Administration, Uppsala, Sweden. firstname.lastname@example.org
The gastrointestinal absorption and organ distribution of Cd after
exposure for 9 weeks to three fibre-rich foodstuffs (wheat bran, sugar-beet
fibre and carrots) were determined in mice. Groups of eight mice were given
a diet containing 0.05 mg Cd/kg from wheat bran, sugar-beet fibre, carrots or
CdCl2 mixed in a semi-synthetic, low-Cd (< 0.007 mg/kg) feed. A control group
was fed on the low-Cd semi-synthetic feed. The water consumption, food
consumption and the weight of the animals were monitored throughout the study.
The feed was changed once weekly and Cd was analysed in the feed at each change.
myo-Inositol phosphates (hexa-, penta-, tetra- and tri-) and Zn, Cu, Fe and Ca
were also analysed in the diets. After 9 weeks, the mice were killed and liver
and kidneys were sampled and analysed for Cd. The group receiving the
wheat-bran diet had significantly lower fractional Cd accumulation (% total Cd
intake) in the liver and kidneys than the other groups, indicating a lower
fractional absorption of Cd. The wheat-bran diet had markedly higher levels
of inositol hexa- and pentaphosphates (phytates) and a Zn level that was twice
as high as those in the other diets. The higher levels of myo-inositol hexa- and
pentaphosphates in the wheat-bran diet most probably contributed more to the
lower fractional absorption of Cd than the elevated Zn level, due to the
formation of insoluble Cd-phytate complexes. Compared with the wheat-bran
diet, the sugar-beet-fibre and carrot diets contained very low levels of myo-inositol
penta- and hexaphosphates, and consequently the fractional Cd absorption from
these diets was higher.
The above study suggested to me that cadmium may
accumulate in the liver and kidneys of animals and it might be wise to
avoid eating these organs to minimize your cadmium intake. The following
study supports this idea. In a pristine area of Canada, eating liver and
kidneys from moose kidney and liver can greatly increase dietary cadmium
intake. Also note that in this relatively environmentally pure area, the
cadmium intake was estimated to be about 136 micrograms per week. Of
this amount only about 16 micrograms is from smoking tobacco (but this
may be an average of smokers and non-smokers). Selenium is used to
eliminate cadmium, so removing 136 mcg of cadmium a week will use up at
least 193 mcg of selenium a week, or about 28 mcg per day. Considering
that other toxic metals like mercury also use up selenium for
detoxification, the drain on selenium can be very significant. In a more
polluted area where selenium is low in the food supply, selenium
deficiency could easily result. Conclusion: avoid eating kidney and
liver to keep cadmium intake low.
|Food Addit Contam 1998 Apr;15(3):307-17
Risk assessment of cadmium exposure in Fort
Resolution, Northwest Territories, Canada.
Kim C, Chan HM, Receveur O.
Centre for Indigenous Peoples' Nutrition and the Environment (CINE),
McGill University, Ste-Anne-de-Bellevue, Quebec, Canada.
The aim of this study is to investigate the cadmium (Cd) exposure
level from traditional food in Fort Resolution, Northwest Territories.
We used 24-h dietary recalls and traditional food use frequency to
obtain information on traditional food consumption, and analysed
cadmium concentrations in traditional food. We also estimated total
cadmium intake via market and traditional food, and cigarette smoking.
Traditional food accounted for only 10% of the dietary energy. About
30 species of wildlife and plants were used. The most important foods
in the community were moose, caribou, and whitefish. The range of
cadmium concentrations measured was 0 to 1869 micrograms/g wet weight,
with the lowest found in cranberry, and the highest in moose kidney.
Cadmium concentrations in traditional food groups were comparable with
those of Canadian market food. Highest levels of cadmium were found in
the liver and kidney of caribou and moose. Cadmium intakes from
traditional food estimated by dietary recall ranged from 0.01 to 1713
micrograms/g/day/person. Average cadmium intakes for women and men
from traditional food were estimated to be 9% and 6% respectively, of
the Provisional Tolerable Weekly Intake (PTWI, 400-500 micrograms)
established by the World Health Organization. The average cadmium
inhaled from cigarette smoking was 2.31 +/- 1.00
micrograms/day/person. There was no difference between the total
cadmium intakes of smokers and nonsmokers. The total cadmium intake
via market and traditional food and cigarette smoking was 136.6
micrograms/ week, which was lower than the PTWI. However, about 20% of
the population may consume caribou and moose organ more frequently
than the others. The potential health effects on this sub-population
needs further clarification.
- The following study suggests that the maximum
dietary intake of cadmium should be 30 mcg per day (210 mcg per
week). When you compare the average intake in the prior study, the
average person living in northern Canada is dangerously close to the
point where toxicity will occur. This means that many people in that
area and in more industrial areas are consuming toxic amounts of
|Br J Nutr 2000 Dec;84(6):791-802
Safe levels of cadmium intake to prevent renal
toxicity in human subjects.
Satarug S, Haswell-Elkins MR, Moore MR.
National Research Centre for Environmental Toxicology and Indigenous
Primary Health Program, The University of Queensland, 39 Kessels Road,
Coopers Plains, Brisbane 4108, Queensland, Australia. email@example.com
The present review attempts to provide an update of the scientific
knowledge on the renal toxicity which occurs in human subjects as a
result of chronic ingestion of low-level dietary Cd. It highlights
important features of Cd toxicology and sources of uncertainty in the
assessment of health risk due to dietary Cd. It also discusses potential
mechanisms for increased susceptibility to Cd toxicity in individuals
with diabetes. Exposure assessment on the basis of Cd levels in
foodstuffs reveals that vegetables and cereals are the main sources of
dietary Cd, although Cd is also found in meat, albeit to a lesser
extent. Cd accumulates particularly in the kidney and liver, and hence
offal contains relatively high amounts. Fish contains only small
quantities of Cd, while crustaceans and molluscs may accumulate larger
amounts from the aquatic environment. Data on Cd accumulation in human
kidney and liver obtained from autopsy studies are presented, along with
results of epidemiological studies showing the relationship between
renal tubular dysfunction and kidney Cd burden. These findings suggest
that a kidney Cd level of 50 microg/g wet weight is a maximum tolerable
level in order to avoid abnormal kidney function. This renal Cd burden
corresponds to a urinary Cd excretion of 2 microg/d. Accordingly, safe
daily levels of Cd intake should be kept below 30 microg per person.
Individual variations in Cd absorption and sensitivity to toxicity
predicts that a dietary Cd intake of 30 microg/d may result in a slight
renal dysfunction in about 1% of the adult population. The previous
guideline for a maximum recommended Cd intake of 1 microg/kg body weight
per d is thus shown to be too high to ensure that renal dysfunction does
not occur as a result of dietary Cd intake.
The following study corroborates data that indicates that
green leafy vegetables have a higher cadmium content than most foods such as
grains. Interestingly, soy beans were found to be much higher in cadmium
than other beans. Another reason not to eat soy.
Sci Total Environ 1998 Sep 18;220(2-3):137-45
Lead and cadmium contents in cereals and pulses in
Zhang ZW, Watanabe T, Shimbo S, Higashikawa K, Ikeda M
Department of Public Health, Kyoto University, Faculty of Medicine, Kyoto,
It is known that, unlike Japanese, Koreans or southern Chinese who depend on
rice as a major source of energy for daily life, people in north-eastern China
rely not only on rice, but on wheat and other cereals and to a lesser extent
also on pulses. Cereal and pulse samples were collected from open markets in
north-eastern China, and analyzed by inductively-coupled plasma spectrometry (ICP-MS)
for two potentially hazardous heavy metals--lead (Pb) and cadmium (Cd). The
average Pb level in cereals (31.3 ng Pb/g as a geometric mean) and that of
pulses (25.7 ng Pb/g) were similar to each other with no significant difference.
Among the cereals, Pb contents were higher in foxtail millet (54.3 ng/g) and
lower in maize (35.4 ng Pb/g; grain and flour in combination), wheat flour (28.8
ng Pb/g) and rice flour (22.7 ng Pb/g). Lead levels in two important types of
pulses, kidney bean and soybean (24.6 and 30.8 ng Pb/g, respectively), were
comparable to the levels in rice and wheat. In contrast, Cd levels were
substantially higher in pulses (55.7 ng Cd/g) than in cereals (9.2 ng Cd/g), and
among the pulses, Cd in soybean (55.7 ng Cd/g) was significantly higher than
that in kidney bean (23.8 ng Cd/g). The possible public health implication
of the Pb and Cd levels, especially the high Pb level in foxtail millet (54.3 ng
Pb/g) and the high Cd level in soybean (73.5 ng Cd/g), is discussed.
Germany is an industrialized country with high rates of
thyroid disease. The following study shows the relative concentrations of
cadmium in various foods in Germany. As expected, some lettuce samples
showed high levels of cadmium, possibly as a result of using sewage sludge as a
fertilizer. Surprisingly to me, breads, cakes, and pastries were also
quite high in cadmium. I am wondering if the grain milling process or some
other mechanical process introduces cadmium. While most meats are low in
cadmium, liver and kidney are particularly high.
Food Addit Contam 1996 Apr;13(3):359-378
Oral cadmium exposure of adults in Germany. 1: Cadmium
content of foodstuffs and beverages.
Muller M, Anke M, Hartmann E, Illing-Gunther H
Friedrich Schiller University Jena, Biological-Pharmaceutical Faculty,
Institute of Nutrition and Environment, Germany.
The cadmium contents of 94 and 105 foodstuffs bought in six-fold repetition
in 1988 and in nine-fold repetition in 1991, respectively were analysed within
the framework of a market-basket study. These foodstuffs were typical of German
eating habits. Additionally, 170 samples of drinking water were investigated.
The cadmium concentrations of the foodstuffs were comparable with results of
recent studies carried out in Europe and North America. Fruit, milk and dairy
products, sugar and sugar-rich foodstuffs as well as beverages showed mean
cadmium contents < or = 5 ng/g fresh matter or ng/ml, respectively. The
cadmium content of meat, sausage, fish and tinned fish was also low. Pork and
beef, the most important kinds of meat, contained 5.4 and 2.5 ng/g on average.
The majority of the vegetables investigated, including potatoes, had cadmium
concentrations < 25 ng/g. However, individuals samples of lettuce showed
very high cadmium levels. The cadmium content of bread, cakes and
pastries as well as farinaceous products were within the range of 20-40 ng/g.
The most important bread, cakes and pastries (wheat and rye bread, toasted
bread, rolls) contained 25-35 ng/g. A median cadmium concentration of 0.2
micrograms/l was found in the drinking water. As expected, liver and kidneys
showed the highest cadmium levels of 73 and 204 ng/g, respectively on average.
Another study on cadmium in bread.
Title: Lead and cadmium contents in Finnish breads.
Tahvonen R, Kumpulainen J
Agricultural Research Centre of Finland, Central Laboratory, Jokioinen.
A total of 647 breads from nine bakeries were pooled into 48
representative samples. After wet digestion in concentrated HNO3, the
contents of lead and cadmium were determined using electrothermal atomic
absorption spectrometry with Zeeman effect background correction and
(NH4)H2PO4 matrix modification. The mean lead and cadmium contents found
in the various types of bread were: rye bread 16 and 14, coffee bread 19
and 23, French bread 8 and 27, whole wheat bread 8 and 30, mixed bread
group I 17 and 27, and mixed bread group II 14 and 28 micrograms/kg. The
mean and median lead contents of all breads were 14 and 8 micrograms/kg.
The samples showed a very high variation in their lead contents. In the
present study, the lead content found in Finnish breads was much lower
than that in the late 1970s. The mean and median cadmium contents in all
of the bread samples analysed were 24 and 25 micrograms/kg. The cadmium
content of rye breads was clearly lower than that of the other bread types
studied. The content of cadmium in the different types of bread was at
about the same level as that reported in Finnish breads in the late 1970s.
The title of the following study indicates that coffee and tea may contain significant
amounts of cadmium, however there is no abstract.
Title: Cadmium and cobalt in tea and coffee and their
relationship to cardiovascular disease.
Horwitz C, Linden SE van der
PMID: 4814501, UI: 74107894
Carrots are high in cadmium (and lead) but steps can
be taken to reduce the cadmium content: pealing the skin, soaking in
water, and cooking all reduce the cadmium content. It may be a good
idea to avoid carrots and carrot juice if you have thyroid disease.
Rocz Panstw Zakl Hig 1997;48(2):187-92
[The influence of culinary processing on content of lead
and cadmium in carrots].
[Article in Polish]
Wieczorek C, Kostrzewa M
Zaklad Technologii Gastronomicznej, Wydzial Zywienia Czlowieka oraz
Gospodarstwa Domowego, Warszawa.
The research was performed to determine both safety of carrot dishes
consumption taking into account lead and cadmium intake and prospect of lowering
content of these elements in carrot during culinary processing. Different but
unspecified varieties of investigated carrot came from the Warsaw market and
from allotments and fields in Upper Silesia. Lead and cadmium level was
determined in raw material as well as the elements' distribution in different
parts of carrot root. The impact of initial treatment including peeling and
soaking in water of the crumbled vegetable was studied. The carrot was cooked
traditionally beginning with boiling or cold water. Lead and cadmium content
were determined using atomic absorption spectrophotometry for extracts of APDC
complexes. The investigated raw material contained little lead (an average 0.019
mg/kg) and cadmium (average 0.015 mg/kg). Both elements are distributed in
layers in entire carrot root. The highest concentration of both lead and
cadmium can be found in the skin (0.075 mg Pb and 0.0115 mg Cd/kg skin), lower
in parenchyma (0.028 mg Pb and 0.066 mg Cd/kg parenchyma) and the lowest in the
core (0.027 mg Pb and Cd/kg core). Because of unequal concentration of both
elements in each layer, peeling eliminates up to 25% lead and about 19% cadmium.
Keeping carrot in water showed a tendency to diminish the level of both elements
in raw material. Soaking carrot for 24 h made it shed 40% of lead and 67% of
cadmium. Cooking process decreased both metal's concentration in the vegetable.
The traditional cooking method diminished lead content by 6-47% and cadmium
content by 35-44%.
- Chocolate is high in copper but for some reason
it does not seem to be a good food for hypers which the high copper
content would suggest. Because of this and other reasons, I
developed a suspicion that chocolate is high in cadmium.
Searching for medical studies on cadmium and chocolate led me to
only two studies. The first study below is very suggestive
that my suspicion is correct and that cocoa beans may be high in
both cadmium and lead. The second study confirms that chocolate is
high in cadmium (and also nickel). It's possible that the cadmium is
introduced to the cocoa during processing, possibly by contact with
galvanized containers, and is not natural to the food.
Lead and cadmium content in cocoa beans (short
Prugarova A, Kovac M
Food Research Institute, Bratislava, Czechoslovakia.
The choice of cocoa beans as the experimental and sample material for
study of the contamination with lead and cadmium was inspired by high Pb
and Cd limits in foods made on its basis (cocoa powder, chocolate) as
well as by the relatively high proportion of these foods in human
nutrition. For Cd, the limits in food products are within the range of
0.01 mg X kg-1 (milk) to 1.0 mg X kg-1 (kidneys) whereas the limits for
lead range between 0.1 mg X kg-1 (e.g. milk) and 10.0 mg X kg-1 (e.g.
tea, yeast, crustaceans, molluscs). Limits for Pb and Cd in foods made
on cocoa bean basis are given in Table 1.
|Food Addit Contam 1994 May-Jun;11(3):351-63
Beverages as a source of toxic trace element
Pedersen GA, Mortensen GK, Larsen EH
National Food Agency of Denmark, Central Laboratory, Soborg.
Beverages of different kinds have been investigated for their content
of lead, cadmium, nickel, chromium, arsenic and mercury. About a ten
times higher lead concentration was found in wine than in most other
beverages. Cocoa was high in cadmium and nickel and some vegetable
juices contained high levels of nickel. The daily intake of trace
elements from beverages was estimated. Wine was still the most
significant source of lead even if the bottles did not have lead
capsules. By consumption of half a bottle per day the daily intake of
lead would be doubled and it would contribute 12% of Provisional
Tolerable Weekly Intake. Cocoa is an important source of cadmium and
nickel, and consumption of tea as well as vegetable juices could
increase the nickel intake significantly. The data are compared to
Danish maximum limits on lead and cadmium.
MACULAR DEGENERATION AND CADMIUM
The following study states that macular degeneration is the
leading cause of visual impairment in the elderly and that the adverse effect of
smoking (a known inducer of cadmium toxicity) is well established.
J Med Genet 2000 Feb;37(2):83-7
Genetic susceptibility to age related macular
Yates JR, Moore AT
Department of Medical Genetics, University of Cambridge, Box 134,
Addenbrooke's Hospital, Cambridge CB2 2QQ, UK.
Age related macular degeneration (AMD) is the leading cause of visual
impairment in the elderly and a major cause of blindness in the developed world.
The disease can take two forms, geographic atrophy and choroidal
neovascularisation. The pathogenesis of AMD is poorly understood. There are
undoubtedly environmental and other risk factors involved and the adverse effect
of smoking is well established. Several studies have shown that genetic
factors are important but leave uncertainty about the magnitude and nature of
the genetic component and whether it varies with the type of AMD. Several
hereditary retinal dystrophies show similarities to AMD and these genes are
potential candidate susceptibility genes. Particular interest has focused on the
ABCR gene which is responsible for autosomal recessive Stargardt macular
dystrophy. It has been claimed that heterozygotes for ABCR mutations are
predisposed to AMD but the data are conflicting. Studies of the genes
responsible for autosomal dominant Sorsby fundus dystrophy, Doyne honeycomb
retinal dystrophy, and Best disease have given negative results. In one large
AMD family, linkage has been reported to markers in 1q25-q31. Recent data
suggest that the ApoE epsilon4 allele may be associated with reduced risk of
AMD. A better understanding of the genetic factors in AMD would contribute to
understanding the pathogenesis. If those at risk could be identified it may be
possible to modify lifestyle or develop novel therapies in the presymptomatic
stage to prevent disease or decrease its severity.
Following is an excellent twin case study on the effects of
smoking on thyroid disease. The key conclusions are that cumulative
cigarette smoking is associated with autoimmune thyroid disease, particularly
Graves' Disease with ophthalmopathy, and that smoking (think cadmium) leads to
autoimmune Graves' Disease when iodine intake is adequate and autoimmune
Hashimoto's Thyroiditis when iodine intake is inadequate. Particularly
when we factor in other sources of cadmium toxicity, this is very powerful
evidence that cadmium is the prime source of autoimmune thyroid disease.
Cigarette Smoking and Risk of Clinically Overt Thyroid Disease
A Population-Based Twin Case-Control
Thomas Heiberg Brix, MD; Pia Skov Hansen, MD; Kirsten
Ohm Kyvik, MD, PhD; Laszlo Hegedüs, MD
Background The effects of cigarette smoking on the thyroid gland have
been studied for years. However, the effect of smoking on thyroid function and
size is still controversial.
Objective To determine the impact of cigarette smoking on the development
of clinically overt thyroid disease.
Methods Matched case-control study of 132 same-sex twin pairs (264
individuals) discordant for clinically overt thyroid disease, ascertained from a
population-based nationwide twin register. Information on thyroid disease and
smoking habits was gathered by questionnaire, and the patients' endocrinologist
or general practitioner verified the diagnosis.
Results Overall, smoking was associated with an increased risk of
developing clinically overt thyroid disease (odds ratio, 3.0; 95% confidence
interval, 1.4-6.6; P=.003). This association remained statistically
significant in monozygotic and dizygotic disease-discordant pairs. The effect of
smoking was more pronounced in monozygotic vs dizygotic pairs (odds ratio, 5.0
vs 2.5; P=.04 for both). Essentially similar results were obtained after
subdividing the twin pairs into groups discordant for clinically overt
autoimmune (49 pairs) and nonautoimmune (83 pairs) thyroid disease. Among twin
pairs concordant for smoking, probands with clinically overt autoimmune thyroid
disease smoked significantly more than did their healthy co-twins (17 pairs; P=.03),
whereas no difference was found between probands with nonautoimmune thyroid
disease and their healthy co-twins (34 pairs; P=.20).
The most striking effect of smoking on the thyroid is its strong
association with Graves ophthalmopathy and Graves disease without
ophthalmopathy, although the latter association is weaker. Although previous
studies have repeatedly shown a clear association between smoking and Graves
disease, with and without ophthalmopathy, they have come to somewhat different
conclusions regarding a possible correlation between smoking severity and the
prevalence or severity of Graves disease. In the present study, we found that,
among twin pairs concordant for smoking, probands with clinically overt Graves
disease smoked significantly more than their healthy co-twins. This finding is
consistent with results from other studies demonstrating
a positive correlation between cumulative cigarette consumption (counted in
pack-years) and the development of Graves disease in genetically susceptible
individuals. Such a correlation could not be demonstrated in clinically overt
nonautoimmune thyroid disease, indicating that a dose effect of smoking is a
risk factor in autoimmune thyroid disease, but not in thyroid disease in
In fact, recent evidence indicates that the predominant effect of smoking on the thyroid is goitrogenic
or antithyroid when iodine intake is low, and immunogenic when intake is
adequate. In our study, all participants were residents of Denmark, an area with
borderline iodine deficiency but no endemic goiter.
Conclusions Smoking is associated with an increased risk of developing
clinically overt thyroid disease. Furthermore, our data suggest that
cumulative cigarette consumption is a risk factor, most pronounced in autoimmune
Arch Intern Med. 2000;160:661-666
Information contributed by Christine Cline:
Since John has made us aware of a cadmium connection, I decided to check into it. I found this info in the 1996 edition of the
"Cadmium is a toxic trace mineral that has many similarities to zinc. There is no biological function for this element in humans. Its toxic effects are kept under control in the body by the presence of zinc.
"Food refining processes disturb the important cadmium-zinc balance.
In WHOLE wheat, cadmium is present in proportion to zinc in a ratio of 1 to 20.
"Cadmium is found primarily in refined foods such as flour, rice, and white sugar. It is present in the air in cigarette smoke and in air pollution such as found around zinc factories. In addition, soft
water usually contains higher levels of cadmium than does hard water. Soft water, especially if it is acid, leaches cadmium from metal
"The liver and kidneys are storage areas for both cadmium and zinc. The total body concentration of cadmium increases with age and varies in different areas of the world. It is very poorly
absorbed, so in normal dietary and environmental circumstances should not be a problem.
"When a deficit of zinc occurs in the diet, the body may make it up by storing cadmium instead. If the daily intake of zinc is high, zinc
will be stored and cadmium will be excreted.
-- "Cadmium also interferes with the absorption of copper."
-- "Zinc is a natural antagonist to cadmium."
-- "When zinc antagonizes cadmium, protection from cancer may occur."
-- "Cadmium can be detoxified with selenium."
-- "Alginates (found in seaweed) bind to cadmium and remove it from the body, a process which could prevent poisoning."
"Cadmium poisoning is a very subtle process and can accumulate over a lifetime. Cadmium deposits in the kidneys, causing kidney damage,
and settles into arteries, raising blood pressure and resulting in
"Cigarette smoke contains substantial amounts of cadmium. One pack of cigarettes deposits 2 to 4 micrograms into the lungs of a smoker
while some of the smoke passes into the air to be inhaled by smokers and nonsmokers alike."
I think John is on to something with this cadmium subject. I'm unable to find info that relates it directly to the thyroid, but that doesn't mean the info is not out there. Cadmium is directly related
to copper and zinc--and copper and zinc are big players in thyroid function.
Note re smoking:
John states in his new website, under nutrients and toxics: "Maybe one of the reasons people smoke is to get cadmium into their bodies."
I've read in thyroid books that some hyperT patients START smoking AFTER they are diagnosed with Graves'. I can testify to that myself.
Was diagnosed at age 29 and started smoking cigarettes for the first time when I was 31.
I still smoke (yeah I know, I know), but I had figured it didn't CAUSE my Graves' because I had the disease before I started smoking. But now that I have learned about how cadmium accumulates in the tissues,
and how cadmium poisoning can accumulate slowly over time..... well, I may need to re-think that one.
And I've avoided all forms of seaweed, which bind to cadmium and remove it from the body. Maybe cadmium overload may be one of the reasons I can't sustain remission.... I learn so much more EVERY
This article first
appeared in the
issue of VRP's Nutritional News
The following study about cadmium toxicity
talks about the disease Itai-Itai, which is a disease of cadmium
toxicity characterized by osteoporosis.
by Al Adrian
is a green, fresh water algae widely distributed throughout the
biosphere, and which possesses unique nutritional properties. Used in
Japan since its culture became commercially and technically feasible in
the early 60s, chlorella is now Japan's most popular food supplement
with over 1,000 tons produced annually.
Chlorella has many
interesting nutritional properties. It contains more chlorophyll per
gram than any other plant. For comparison, Spirulina contains 7.6 gm of
chlorophyll per kilogram, whereas Chlorella contains 20-25 gm per
kilogram. In addition, dehydrated Chlorella powder is from 50 to 60
percent protein, and is a significant source of beta-carotene, RNA, and
vitamin B-12 (nearly 1.5 times that found in beef liver and therefore an
excellent supplement for vegetarians). Chlorella powder also contains
vitamin C, vitamin B-1, vitamin B-2, vitamin B-3, vitamin B-5, vitamin
B-6, folic acid, and biotin.
However, the real benefit
of Chlorella does not lie in consumption of traditionally well defined
nutrients such as protein and the B vitamins from a novel source, but
rather in consumption of a product which has been shown to have
antiviral, wound-regenerating, immune-enhancing, and detoxification
properties. Japanese biochemists have isolated an ill-defined water
soluble extract of
peptides) dubbed CGF or Chlorella Growth Factor. Studies in Japan have
found CGF to have anti cancer effects in standard cancer rodent models.
Furthermore, tests of an acidic polysaccharide extract of Chlorella cell
walls have demonstrated interferon induction both in-vitro and in-vivo,
and this material intra-peritoneally has been used to produce
substantial protection of mice to surgically implanted tumors and
infection with influenza virus. This effect on interferon production
probably accounts for a 1966 Japanese study which found that two grams
of daily chlo rella consumption reduced the frequency of colds by 26.5%.
Chlorophyll, well known
for its function in photosynthesis in plants, has also been researched
for its healing properties in humans. Chlorophyll and chlorella have
been found to stimulate the regenera tion of tissues. This effect has
been studied and exploited in the treatment of a variety of wounds and
ulcerative conditions. Topical or oral administration of Chlorella has
been found to be useful in the treatment of diabetic skin ulcers,
pyorrhea, cervicitis, radiation burns, and pancreatitis. The mechanism
by which chlorella speeds healing is under investigation, but it is
hypothesized that cell wall polysaccharides stimulate interferon
production, this in turn stimulates TNF (tumor necrosis factor) which
activates fibroblast activity. This, along with inhibition of proteases
released during inflammation results in a synergistic effect on the
Lastly, but perhaps most
importantly, is Chlorella's growing reputation as a dietary
detoxification agent. Chlorella's detoxification capabilities have been
extensively studied in Japan where it has been found to increase
excretion of cadmium from people suffering from "Itai-itai"
(cadmium poisoning). Aside from Chlorella's detoxification of heavy
metals such as cadmium, uranium and lead, Chlorella cell wall material
has also been found to reduce substantially the half-life of syntheti
cally produced hydrocarbons such as PCBs (polychlorinated biphenyls) and
chlordecone (a pesti cide) in rats under experimental conditions. Dr.
Ueda of the Kitakyushu City Institute for Environ mental Pollution
Research in Japan prescribes a daily consumption of four to six grams of
chlorella powder in the event of PCB (polychlorinated biphenyls)
poisoning--he reports success with the use of this protocol in almost
all of those treated.
product VRP is introducing has been approved for use in Japan for many
years and has some unique advantages. Grown indoors under controlled
conditions, VRP's chlorella is free of the filth, contaminating
microorganisms, insect larvae and pesticides often found in the cultures
of species of chlorella grown outdoors. Also, this particular species of
Chlorella, C hlorella regularis, yields a digestibility value
often exceeding 75%.
In conclusion, although
research on the health benefits of chlorella is on-going, and its
mechanisms of action are still not completely understood,
supplementation with Chlorella on a daily basis is safe and well
tolerated (some cases of allergy have been reported). With daily
production of pesticides and other synthetically produced chemicals
increasing (with accumulation of these substances at the top of the food
chain), daily Chlorella supplementation should be of increasing interest
to the health conscious consumer.
|Toxicol Appl Pharmacol 2000 May 1;164(3):264-72
Uncoupling between bone formation and resorption in
ovariectomized rats with chronic cadmium exposure.
Uriu K, Morimoto I, Kai K, Okazaki Y, Okada Y, Qie YL, Okimoto N, Kaizu
K, Nakamura T, Eto S
Kidney Center, First Department of Internal Medicine, Department of
Orthopedic Surgery, University of Occupational and Environmental Health
School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807, Japan.
[Medline record in process]
Osteoporosis, osteomalacia, and pathological fractures are characteristic
features of Itai-Itai disease. The mechanisms of bone damage caused by
cadmium (Cd) exposure have not been fully clarified. We investigated
skeletal changes in ovariectomized rats with chronic Cd exposure, using bone
histomorphometry and mechanical tests. Female Sprague-Dawley rats at the age
of 8 weeks were ovariectomized. Eight weeks after ovariectomy, the rats were
divided into two groups: Cd-OVX group (n = 15), ovariectomized rats given
cadmium chloride (CdCl(2), 0.18 mg/rat) ip three times a week for 28 weeks;
Cont-OVX group (n = 10), ovariectomized rats given distilled water alone for
28 weeks. Cd-OVX rats had a significant increase in serum concentration of
intact osteocalcine and showed numerical but not significant increase in
urinary excretion of deoxypyridinoline despite a significant decrease in
glomerular filtration rate to 40% of the value in Cont-OVX rats. Bone
mineral content (BMC) and density were significantly decreased in both the
lumbar vertebral body and femur of Cd-OVX rats. Ultimate compressive load in
the lumbar body and bending load in the midfemur were significantly lower in
Cd-OVX rats than in Cont-OVX rats but the differences were not demonstrated
when the values were corrected for BMC. Structural moduli in the lumbar
vertebral body and the midfemur were not different between the two groups.
Cd-OVX rats showed significant decreases in the trabecular bone volume and
trabecular number with increased values in the indices of bone formation and
resorption in the lumbar vertebral body cancellous bone in comparison with
Cont-OVX rats. In the midfemur, Cd-OVX rats had significantly smaller
cortical bone area than Cont-OVX rats but the moment of inertia was
identical between the two groups. The indices of bone formation and
resorption at endocortical surface of the midfemur were significantly
increased in Cd-OVX rats over those in Cont-OVX rats, whereas the indices of
bone formation at the periosteal surface were not different between the two
groups. These data suggested that chronic Cd exposure exacerbated the
uncoupling between bone formation and resorption in ovariectomized rats,
which resulted in the osteopenia, structural changes of the bone, and
decreased mechanical strength in ovariectomized rats with chronic Cd
exposure. Copyright 2000 Academic Press.
The following study makes me wonder if the ear ringing experienced in
thyroid disease is related to cadmium toxicity.
|Hear Res 1999 Mar;129(1-2):61-70
The effect of combined administration of cadmium and
furosemide on auditory function in the rat.
Whitworth CA, Hudson TE, Rybak LP
Southern Illinois University School of Medicine, Department of Surgery,
Springfield 62794-1221, USA.
A number of heavy metals have been associated with toxic effects to the
peripheral or central auditory system. These include lead, arsenic, mercury,
platinum and organic tin compounds. In addition, the ototoxic effects of
some metals may be potentiated by other factors. However, the auditory
effects of cadmium have not previously been reported. The purpose of the
present study was to investigate the potential ototoxic effects of cadmium
from an acute dosage, and its potentiation by furosemide. Auditory brainstem
response (ABR) thresholds were measured in adult Sprague-Dawley rats. Rats
received either cadmium chloride (5 mg/kg, i.p.) followed by saline (4
ml/kg, i.p.). cadmium chloride followed by furosemide (200 mg/kg, i.p.), or
furosemide alone. Follow-up ABRs were carried out 7 days post-treatment and
threshold changes were compared between each treatment group. No significant
threshold change was seen for the cadmium chloride plus saline treated or
the furosemide treated animals. However, significant threshold elevations
were observed in animals receiving cadmium chloride plus furosemide. In
addition, scanning electron microscopy revealed extensive hair cell loss in
animals treated with cadmium chloride and furosemide. Although functional
auditory changes were not seen after the administration of cadmium alone,
the potentiation of threshold changes by furosemide suggests that cadmium
may be ototoxic under certain conditions.
The following study shows that cadmium has a stronger effect in inducing
selenium and vitamin E deficiencies than 11 other minerals.
|Am J Vet Res 1982 May;43(5):851-7
Amounts of twelve elements required to induce
selenium-vitamin E deficiency in ducklings.
Van Vleet JF
Mortality and myopathy of selenium-vitamin E (Se-E) deficiency was produced,
in a concentration-dependent pattern, during a 4-week study of 750 ducklings
fed a commercial duck starter mash that contained adequate amounts of Se and
E, and supplemented with multiple amounts of Ag (50 to 3,000 mg/kg of feed,
as acetate), Zn (3,000 to 6,000 mg/kg, as sulfate), Cd (10 to 500 mg/kg, as
sulfate), Te (25-500 mg/kg, as tetrachloride), Co (100 to 1,000 mg/kg, as
chloride), Cu (500 to 1,500 mg/kg, as sulfate), Hg (200 to 400 mg/kg, as
chloride), and Sn (1,000 mg/kg, as chloride). Also, feeding supplements of
Pb (500 mg/kg, as acetate), As (600 mg/kg, as sodium arsenilate), Fe (5,000
mg/kg, as sulfate), and S (5,000 mg/kg, as sodium sulfite) produced a
low-to-medium frequency of lesions of Se-E deficiency. In ducklings with
muscle lesions, the gizzard was most often affected (84.2%), followed in
decreasing order by skeletal muscles (69.7%), intestine (34.9%), and heart
(23.0%). The frequency of skeletal muscle lesions was high in birds fed Ag,
and myocardial necrosis was frequent in ducklings fed Te and Hg. Ducklings
affected with myopathy were reluctant to stand. Subcutaneous edema, with or
without hemorrhages, and pale areas of myonecrosis in gizzard, skeletal
muscles, intestine, and heart were seen at necropsy. Birds fed Te and Hg
often had hydropericardium and hemorrhagic myocardial necrosis. Seemingly,
addition of many elements to a Se-E adequate commercial diet will increase
the requirement for Se-E. In our duckling model, minimal amounts shown to
induce Se-E deficiency were 50 mg of Ag/kg, 3,000 mg of Zn/kg, 10 mg of Cd/kg,
25 mg of Te/kg, 1200 mg of Co/kg, 500 mg of Cu/kg, 200 mg of Hg/kg, 1,000 mg
of Sn/kg, 500 mg of Pb/kg, 600 mg of As/kg, 5,000 mg of Fe/kg, and 5,000 mg
The following study shows that cadmium accumulates in the thyroid more
than most other areas of the body. Perhaps this indicates that cadmium has a
role in thyroid function.
Environ Res 2000 Nov;84(3):211-8
Mercury, selenium, and cadmium in human autopsy samples
from idrija residents and mercury mine workers.
Falnoga I, Tusek-Znidaric M, Horvat M, Stegnar P
Department of Environmental Sciences, Jozef Stefan Institute, Jamova 39,
Ljubljana, 1111, Slovenia.
[Medline record in process]
Total Hg and Se concentrations were determined in autopsy samples of retired
Idrija mercury mine workers, Idrija residents living in a Hg-contami-nated
environment, and a control group with no known Hg exposure from the environment.
In selected samples we also checked the presence of MeHg. The highest Hg
concentrations were found in endocrine glands and kidney cortex, regardless of
the group. MeHg contributed only to a negligible degree to the total mercury
concentrations in all analyzed samples. In the Hg-exposed groups the
coaccumulation and retention of mercury and selenium was confirmed. Selenium
coaccumulation with a Hg/Se molar ratio near 1 or higher was notable only in
those tissue samples (thyroid, pituitary, kidney cortex, nucleus dentatus) where
the mercury concentrations were >1 &mgr;g/g. After tissue separation of
such samples the majority of these elements were found in the cell pellet.
Because the general population is continuously exposed to Cd and possibly also
to Pb from water, food, and/or air, in some samples the levels of these elements
were also followed. In all examined control tissue samples the average values of
Cd (kidney cortex, thyroid, hippocampus, cortex cerebellum, nucleus dentatus)
and Pb (thyroid, hippocampus) exceeded the average values of Hg. Cd
concentrations were the highest, particularly in kidney cortex and thyroids
(&mgr;g/g), but no relationship between Cd and Se concentration was evident
at the tissue level. Regarding the results in the control group, it is debatable
which element is the more hazardous for the general population as concerns
neurotoxicity. Copyright 2000 Academic Press.
|J Environ Pathol Toxicol Oncol 2000;19(3):201-13
Oxidative mechanisms in the toxicity of chromium and
Stohs SJ, Bagchi D, Hassoun E, Bagchi M
Department of Pharmaceutical Sciences, Creighton University School of
Pharmacy and Allied Health Professions, Omaha, NE 68178, USA.
Chromium and cadmium are widely used industrial chemicals. The toxicities
associated with both metal ions are well known. However, less information is
available concerning the mechanisms of toxicity. The results of in vitro and
in vivo studies demonstrate that both cations induce an oxidative stress
that results in oxidative deterioration of biological macromolecules.
However, different mechanisms are involved in the production of the
oxidative stress by chromium and cadmium. Chromium undergoes redox cycling,
while cadmium depletes glutathione and protein-bound sulfhydryl groups,
resulting in enhanced production of reactive oxygen species such as
superoxide ion, hydroxyl radicals, and hydrogen peroxide. These reactive
oxygen species result in increased lipid peroxidation, enhanced excretion of
urinary lipid metabolites, modulation of intracellular oxidized states, DNA
damage, membrane damage, altered gene expression, and apoptosis. Enhanced
production of nuclear factor-kappaB and activation of protein kinase C
occur. Furthermore, the p53 tumor suppressor gene is involved in the cascade
of events associated with the toxicities of these cations. In summary, the
results clearly indicate that although different mechanisms lead to the
production of reactive oxygen species by chromium and cadmium, similar
subsequent mechanisms and types of oxidative tissue damage are involved in
the overall toxicities.
The following study seems to indicate that cadmium causes iron deficiency
|Jpn J Vet Res 2000 May;48(1):15-28
Experimental reproduction of itai-itai disease, a
chronic cadmium poisoning of humans, in rats and monkeys.
Department of Veterinary Clinical Sciences, Graduate School of Veterinary
Medicine, Hokkaido University, Sapporo, Japan. firstname.lastname@example.org
To establish a useful animal model of Itai-Itai disease (IID) of humans,
we conducted the following experiments. Experiment 1: Toxic effects of Cd
were compared between ovariectomized (OX) and non-OX rats after daily,
intravenous injection of cadmium (Cd) chloride for 14 days. In this
experiment, we demonstrated that OX rats were more susceptible to Cd-induced
nephrotoxicity and hepatotoxicity than non-OX rats. Experiment 2: OX rats
were injected with Cd at doses of 1.0 and 2.0 mg/kg, 5 days a week, for 13
weeks. The bone Cd content was gradually increased for 13 weeks in a
dose-dependent manner. Calcium and phosphorus contents in the bone and
serum levels of parathyroid hormone and osteocalcin were not significantly
different between Cd-treated and control rats. Mild osteomalacic lesions
in the cortical bones of the midshaft haversian canals as well as chronic
nephropathy appeared in the rats of the 2.0 mg/kg group. Experiment 3: OX
rats were treated with Cd at doses of 0.5 and 0.05 mg/kg for 70 weeks. The
rats of the 0.05 mg/kg group showed slight anemia and mild degeneration of
tubular epithelium after 50 weeks of treatment. In the 0.5 mg/kg group,
the rats showed definite osteomalacia of bones and nephrosclerosis. The Cd
concentration in the bones increased for the first 25 weeks, but was
replaced gradually with iron at from 50 to 70 weeks of the administration
period. Iron deficiency anemia appeared in the 0.5 mg/kg group at from 12
to 25 weeks, and changed to renal anemia after 50 weeks of administration.
The anemia at 50 and 70 weeks was normocytic and normochromic, and serum
erythropoietin levels were not elevated in response to the decrease of
hemoglobin concentrations of red blood cells. Experiment 4: Ten, OX
cynomolgus monkeys were given intravenous injections of 0, 1.0 or 2.5
mg/kg/day Cd, 2 or 3 days per week, for 13 to 15 months. Normocytic and
normochromic anemia, renal lesions characterized by tubular atrophy and
interstitial fibrosis (Cd nephropathy), and bone lesions characterized by
an increase of osteoid and osteopenia (Cd osteopathy) were induced in the
monkeys treated with Cd. These results demonstrated that chronic cadmium
toxicosis similar to IID of humans was reproducible in rats and monkeys by
repeated intravenous injection of Cd and that a disease entity closely
resembling IID of humans could be induced in experimental animals by
chronic Cd toxicosis without participation of malnutrition, vitamin D
deficiency, impaired absorption at the intestinal mucosa or multiparous
|Med Hypotheses 1985 Jul;17(3):231-42
Zinc, cadmium, metallothionein, and progesterone: do they
participate in the etiology of pregnancy induced hypertension?
Chisolm JC, Handorf CR.
Cadmium, a toxic heavy metal, has been incriminated in the etiology of
essential hypertension. Zinc, an essential micronutrient necessary for
growth, competes with cadmium for binding sites in biochemical processes;
zinc deficiency states (i.e. pregnancy and low protein diet) might expose an
individual to increased risk of cadmium toxicity. The increased sensitivity
to cadmium during pregnancy could also be related to the effect of
progesterone on zinc and cadmium metabolism through the actions of
metallothionein (MT). MT is a low molecular weight protein believed to
function in cadmium detoxification. Several studies in lab animals have
documented a late gestation drop of maternal MT levels. This was thought to
be due to rising progesterone levels. If there is also a late gestation drop
in human maternal MT, then the propensity toward maternal cadmium toxicity
would be enhanced. Therefore, we propose that when a zinc deficient woman
becomes pregnant and is exposed to both the nutritional demands of the fetus
and to the influence of progesterone, she will be likely to develop the
manifestations of cadmium toxicity (i.e. hypertension, proteinuria, edema,
- If it is common that members of the brassica family show high cadmium
levels, is cadmium the reason why brassicas are goitrogenic?
|Plant Physiol 1995 Dec;109(4):1427-1433
Mechanisms of Cadmium Mobility and Accumulation in
Salt DE, Prince RC, Pickering IJ, Raskin I.
Center for Agricultural and Molecular Biology, Rutgers University, Cook
College, New Brunswick, New Jersey 08903 (D.E.S., I.R.).
Indian mustard (Brassica juncea L.), a high biomass crop plant,
accumulated substantial amounts of cadmium, with bioaccumulation
coefficients (concentration of Cd in dry plant tissue/concentration in
solution) of up to 1100 in shoots and 6700 in roots at nonphytotoxic
concentrations of Cd (0.1 [mu]g/mL) in solution. This was associated with
a rapid accumulation of phytochelatins in the root, where the majority of
the Cd was coordinated with sulfur ligands, probably as a Cd-S4 complex,
as demonstrated by x-ray absorption spectroscopy. In contrast, Cd moving
in the xylem sap was coordinated predominantly with oxygen or nitrogen
ligands. Cd concentrations in the xylem sap and the rate of Cd
accumulation in the leaves displayed similar saturation kinetics,
suggesting that the process of Cd transport from solution through the root
and into the xylem is mediated by a saturable transport system(s).
However, Cd translocation to the shoot appeared to be driven by
transpiration, since ABA dramatically reduced Cd accumulation in leaves.
Within leaves, Cd was preferentially accumulated in trichomes on the leaf
surface, and this may be a possible detoxification mechanism.