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Table of Contents | |
CADMIUM, ARGININE, NITRIC OXIDE STORY
If you've stumbled onto this page, it may not make a lot of
sense. It doesn't to me yet. However, I have a feeling that there is something
in the connections between cadmium (and maybe bismuth), arginine, and nitric
oxide. Cadmium seems to be a very critical metal in thyroid function and
dysfunction. Nitric oxide is the gas which causes migraines and is also the gas
which causes male erections (hyper sex drive). There are a lot of pieces to put
together and I'm in the process of accumulating studies at this point.
In the first study IBD is a condition which is associated with hyperT.
Inhibition of inducible nitric oxide synthase in the
human intestinal epithelial cell line, DLD-1, by the inducers of heme
oxygenase 1, bismuth salts, heme, and nitric oxide donors.
Cavicchi M, Gibbs L, Whittle BJ
William Harvey Research Institute, St Bartholomew's and the Royal London
School of Medicine and Dentistry, Charterhouse Square, London EC1M 6BQ, UK.
[Medline record in process]
BACKGROUND: The inducible isoform of nitric oxide synthase (iNOS) may be
involved in the mucosal injury associated with inflammatory bowel disease (IBD).
In contrast with iNOS, the inducible heme oxygenase 1 (HO-1) is considered
to act as a protective antioxidant system. AIMS: To evaluate the effects of
the known HO-1 inducers, cadmium and bismuth salts, heme, and nitric oxide
(NO) donors, on iNOS activity, and expression in the human intestinal
epithelial cell line DLD-1. METHODS: iNOS activity was assessed by the
Griess reaction and the radiochemical L-arginine conversion assay. iNOS mRNA
and iNOS protein expression were determined by northern and western
blotting, respectively. RESULTS: Cytokine exposure led to induction of iNOS
activity, iNOS mRNA, and iNOS protein expression. Preincubation of DLD-1
cells with heme (1-50 &mgr;M) inhibited cytokine induced iNOS activity
in a concentration dependent manner. This inhibitory effect was abolished by
the HO-1 specific inhibitor tin protoporphyrin. Preincubation with NO donors
sodium nitroprusside (SNP 1-1000 &mgr;M) or S-nitroso-acetyl-penicillamine
(SNAP 1-1000 &mgr;M), or with the heavy metals cadmium chloride (10-40
&mgr;M), bismuth citrate, or ranitidine bismuth citrate (10-3000 &mgr;M)
inhibited iNOS activity in a concentration dependent manner. Moreover, SNP
and heme abolished cytokine induced iNOS protein as well as iNOS mRNA
expression, whereas cadmium chloride did not modify iNOS protein expression.
CONCLUSIONS: Heme, the heavy metals cadmium and bismuth, as well as NO
donors, are potent inhibitors of cytokine induced iNOS activity. Heme and NO
donors act at the transcriptional level inhibiting iNOS mRNA expression.
Such findings suggest the potential for interplay between the iNOS and HO-1
systems, which may modulate the progress of IBD.
In the following study it is found that cadmium reduces nitric oxide (NO)
formation by inhibiting the enzyme NO synthase. NO decreases blood pressure so
the effect of cadmium in decreasing NO is to cause high blood pressure.
I am thinking now that there is an excess of nitric oxide in hyperT. If
this is true does that mean there may be a deficiency of cadmium in
hyperT? That would be strange.
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Br J Pharmacol 1998 Jan;123(1):129-35 |
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Role of nitric oxide synthase inhibition in the acute
hypertensive response to intracerebroventricular cadmium.
Demontis MP, Varoni MV, Volpe AR, Emanueli C, Madeddu P
Clinica Farmacologia, University of Sassari, Italy.
1. In the rat, intracerebroventricular (i.c.v.) injection of cadmium, a
pollutant with long biological half-life, causes a sustained increase in
blood pressure at doses that are ineffective by peripheral route. Since
cadmium inhibits calcium-calmodulin constitutive nitric oxide (NO) synthase
in cytosolic preparations of rat brain, this mechanism may be responsible
for the acute pressor action of this heavy metal. 2. To test this
possibility, we evaluated the effect of i.c.v. injection of 88 nmol cadmium
in normotensive unanaesthetized Wistar rats, which were i.c.v. pre-treated
with: (1) saline (control), (2) L-arginine (L-Arg), to increase the
availability of substrate for NO biosynthesis, (3) D-arginine (D-Arg), (4)
3-[4-morpholinyl]-sydnonimine-hydrochloride (SIN-1), an NO donor, or (5)
CaCl2, a cofactor of brain calcium-calmodulin-dependent cNOS(I). In
additional experiments, the levels of L-citrulline (the stable equimolar
product derived from enzymatic cleavage of L-Arg by NO synthase) were
determined in the brain of vehicle- or cadmium-treated rats. 3. The pressor
response to cadmium reached its nadir at 5 min (43+/-4 mmHg) and lasted over
20 min in controls. L-Citrulline/protein content was reduced from 35 up to
50% in the cerebral cortex, pons, hippocampus, striatus, hypothalamus
(P<0.01) of cadmium-treated rats compared with controls. Central
injection of N(G) nitro-L-arginine-methylester (L-NAME) also reduced the
levels of L-citrulline in the brain. 4. Both the magnitude and duration of
the response were attenuated by 1.21 and 2.42 micromol SIN-1 (32+/-3 and
15+/-4 mmHg, P<0.05), or 1 micromol CaCl2 (6+/-4 mmHg, P<0.05).
Selectivity of action exerted by SIN-1 was confirmed by the use of another
NO donor, S-nitroso-N-acetyl-penicillamine (SNAP). Both L-Arg and D-Arg
caused a mild but significant attenuation in the main phase of the pressor
response evoked by cadmium. However, only L-Arg reduced the magnitude of the
delayed, pressor response. Despite their similarity in ability to attenuate
the cadmium-induced pressure effect, L-Arg and its isomer exerted
differential biochemical changes in brain L-citrulline, as L-Arg normalized
cadmium-induced reduction in L-citrulline levels, whereas i.c.v. D-Arg did
not. 5. We conclude that the pressor effect of i.c.v. cadmium is due, at
least in part, to reduced NO formation, consequent to inhibition of brain NO
synthase. Accumulation of cadmium in the central nervous system could
interfere with central mechanisms (including NO synthase) implicated in the
regulation of cardiovascular function.
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Toxicol Appl Pharmacol 1996 Dec;141(2):540-7 |
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Metal-induced modulation of nitric oxide production in
vitro by murine macrophages: lead, nickel, and cobalt utilize different
mechanisms.
Tian L, Lawrence DA
Wadsworth Center, Albany, New York 12201-0509, USA.
Macrophages (M phi) can be induced to produce nitric oxide (NO), which has
been suggested to be important for macrophages to exercise various
functions. We have previously reported that an environmental toxicant, lead
(Pb), can significantly inhibit NO production by murine splenic M phis.
Herein, eight additional metal ions, gold (Au), cadmium (Cd), cobalt (Co),
chromium (Cr), copper (Cu), mercury (Hg), nickel (Ni), and zinc (Zn), were
assessed. In addition to Pb, Hg and Cd significantly suppressed NO
production by cytokine (interferon-gamma and tumor necrosis
factor-alpha)-stimulated murine M phis. Au and Cu also were inhibitory, but
less than Pb, Hg, and Cd. In contrast, Cr and Zn were not modulatory, and Ni
and Co significantly enhanced NO production by cytokine-stimulated M phis.
The enhancement by Ni and Co was inhibited by the arginine analog N-monomethylarginine.
The metals showed different activating/inhibiting profiles when added to a
cell-free (activated M phi lysate) NO-producing-system in which inducible NO
synthase (iNOS) is already expressed. Cr, Cu, Pb, and Zn moderately
suppressed iNOS, which suggests that they may directly modify enzyme or
cofactor activity. Cd, Hg, Mg, Ni, or Co did not produce any significant
effect on NO production by the cell-free system. Inhibition of NO production
by Pb-exposed M phis was not due to decreased expression of iNOS nor limited
to its modest direct inhibition of iNOS; thus, other mechanism(s) must be
accountable for the efficient Pb-induced inhibition of NO production by M
phi. Ni or Co did induce a substantial increase of iNOS protein. Overall,
these observations provide additional insight into the means by which metals
via inhibition or enhancement of NO production may be pathogenic, by
suppression of defense mechanisms or induction of hypersensitivity,
respectively.
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Mol Cell Biochem 1995 Aug-Sep;149-150:263-5 |
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Interaction of heavy metal toxicants with brain
constitutive nitric oxide synthase.
Mittal CK, Harrell WB, Mehta CS
Division of Pharmaceutical Sciences, College of Pharmacy and Health
Sciences, Texas Southern University, Houston 77004, USA.
This study was designed to evaluate the in vitro effects of transition heavy
metal cations on activity of constitutive isoform of nitric oxide synthase (cNOS)
in rat brain. NOS activity was determined in the cytosolic fractions of rat
cerebral hemispheres by conversion of 3H-L-arginine to 3H-L-citrulline.
Different concentrations of mercury (Hg2+), nickel (Ni2+), manganese (Mn2+),
zinc (Zn2+), cadmium (Cd2+), lead (Pd2+) and calcium (Ca2+) were tested on
NOS activity. While all the cations caused inhibition, there were
differences in the apparent inhibition constants (Ki) among the cations.
With the exception of calcium ion no other cation required preincubation
with the enzyme preparation. These results indicate that while calcium ion
modulate cNOS activity at regulatory site(s), inhibitory influence of toxic
heavy metal cations may be exerted on the catalytic site(s) either by direct
binding to it or by interfering with the electron transfer during catalysis.
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Biol Trace Elem Res 2000 Nov;77(2):97-106 |
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Tissue and blood levels of zinc, copper, and magnesium in
nitric oxide synthase blockade-induced hypertension.
Senturk UK, Kaputlu 1, Gunduz F, Kuru O, Gokalp O
Department of Physiology, Akdeniz University Faculty of Medicine, Antalya,
Turkey.
[Medline record in process]
The aim of this study was to determine the levels of tissue and blood zinc
(Zn), copper (Cu), magnesium (Mg) in nitric oxide (NO) synthase
blockade-induced hypertension. A group of albino rats received a NO synthase
inhibitor, N(G)-nitro-L-arginine-methyl ester (L-NAME, 60 mg/kg/d) in their
drinking water for 21 d. L-NAME intake caused a progressive rise in this
group's resting mean arterial blood pressure compared to a control group (p
< 0.01). There were no differences between the groups with regard to
tissue and blood levels of Zn or Cu; however, Mg concentrations were
significantly lower in the hypertensive rats' erythrocytes (20.2% reduction
from control levels), cerebral cortex (17.0%), heart (9.1%), renal cortex
(12%), renal medulla (16.7%), and in the tissues of the caval vein (23.7%),
mesenteric artery (29.8%), renal artery (18.4%), and renal vein (22.1%).
There were no significant Mg concentration changes in the hypertensive
group's plasma, cerebellum, liver, duodenum, or aortal tissue. These
findings suggest that Mg depletion may play a role in the blood pressure
rise that occurs in the model of chronic NO synthase inhibition-induced
hypertension.
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J Biol Chem 2000 Jul 14;275(28):21241-6 |
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Inhibitory effects of nitric oxide and nitrosative stress
on dopamine-beta-hydroxylase.
Zhou X, Espey MG, Chen JX, Hofseth LJ, Miranda KM, Hussain SP, Wink DA,
Harris CC
Laboratory of Human Carcinogenesis, Radiation Biology Branch, NCI, National
Institutes of Health, Bethesda, Maryland 20892, USA.
Dopamine-beta-hydroxylase (DbetaH) is a copper-containing enzyme that uses
molecular oxygen and ascorbate to catalyze the addition of a hydroxyl group
on the beta-carbon of dopamine to form norepinephrine. While norepinephrine
causes vasoconstriction following reflex sympathetic stimulation, nitric
oxide (NO) formation results in vasodilatation via a guanylyl cyclase-dependent
mechanism. In this report, we investigated the relationship between NO and
DbetaH enzymatic activity. In the initial in vitro experiments, the activity
of purified DbetaH was inhibited by the NO donor, diethylamine/NO (DEA/NO),
with an IC(50) of 1 mm. The inclusion of either azide or GSH partially
restored DbetaH activity, suggesting the involvement of the reactive
nitrogen oxide species, N(2)O(3). Treatment of human neuroblastoma cells
(SK-N-MC) with diethylamine/NO decreased cellular DbetaH activity without
affecting their growth rate and was augmented by the depletion of
intracellular GSH. Co-culture of the SK-N-MC cells with interferon-gamma and
lipopolysaccharide-activated macrophages, which release NO, also reduced the
DbetaH activity in the neuroblastoma cells. Our results are consistent with
the hypothesis that nitrosative stress, mediated by N(2)O(3), can result in
the inhibition of norepinephrine biosynthesis and may contribute to the
regulation of neurotransmission and vasodilatation.
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Endothelium 2000;7(2):83-92 |
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Endothelial cell calcium mobilization to acetylcholine is
attenuated in copper-deficient rats.
Schuschke DA, Falcone JC, Saari JT, Fleming JT, Percival SS, Young SA,
Pass JM, Miller FN
Center for Applied Microcirculatory Research, University of Louisville
School of Medicine, KY 40292, USA. DASCHU01@GWISE.LOUISVILLE.EDU
Dietary copper deficiency significantly attenuates nitric oxide
(NO)-mediated vascular smooth muscle relaxation and vasodilation. There is
evidence for both increased inactivation of the NO radical by superoxide
anion, and oxidative damage to the endothelium where NO is produced. The
current study was designed to examine the NO synthetic pathway in the
endothelium during copper deficiency. Male weanling rats were fed a
copper-adequate (CuA, 6.4 mg Cu/kg diet) or copper-deficient (CuD, 0.4 mg
Cu/kg diet) diet for four weeks. Cremasteric arterioles (approximately 100
microm diameter) were isolated and used for the experiments. Western blot
analysis of the arteriole endothelial nitric oxide synthase (eNOS)
concentration did not show a difference between dietary groups.
Acetylcholine (Ach)-induced vasodilation was significantly reduced in the
CuD group both before and after pretreatment with the eNOS substrate
L-arginine. Endothelial intracellular calcium ([Ca2+]i) stimulated by 10(-6)
M Ach was significantly inhibited in the arterioles from CuD rats.
Coincident with the inhibition of [Ca2+]i and vasodilation was a depression
of vascular Cu/Zn-SOD activity and an increase in plasma peroxynitrite
activity. These data suggest that endothelial Ca2+ signaling and
agonist-stimulated NO-mediated vascular dilation are likely reduced by
increased oxidative damage in copper-deficient rats.
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J Protein Chem 2000 Jan;19(1):51-7 |
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Arginine and ornithine oxidation catalyzed by lentil
seedling copper-amine oxidase.
Medda R, Padiglia A, Lorrai A, Finazzi Agro A, Floris G
Department of Sciences Applied to Biosystems, University of Cagliari, Italy.
[Medline record in process]
The oxidation of L-ornithine and L-arginine catalyzed by lentil (Lens
esculenta) seedling copper-amine oxidase has been investigated by
polarographic techniques, optical spectroscopy, and capillary
electrophoresis. Both L-ornithine and L-arginine were found to be poor
substrates for lentil amine oxidase. L-Ornithine was oxidized to
glutamate-5-semialdehyde and ammonia, in similar manner as usual substrates.
Glutamate-5-semialdehyde spontaneously cyclizes to
delta1-pyrroline-5-carboxylic acid. Arginine is oxidized by an unusual
mechanism yielding glutamate-5-semialdehyde, ammonia, and urea as reaction
products.
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